Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VPBB_RS13390 Genome accession   NC_019955
Coordinates   2911804..2912382 (-) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus BB22OP     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2906804..2917382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VPBB_RS13365 (VPBB_2602) aroB 2907100..2908200 (-) 1101 WP_015297394.1 3-dehydroquinate synthase -
  VPBB_RS13370 (VPBB_2603) aroK 2908232..2908750 (-) 519 WP_005381319.1 shikimate kinase AroK -
  VPBB_RS13375 (VPBB_2604) pilQ 2908939..2910681 (-) 1743 WP_024699327.1 type IV pilus secretin PilQ family protein Machinery gene
  VPBB_RS13380 (VPBB_2605) pilP 2910713..2911228 (-) 516 WP_015297395.1 pilus assembly protein PilP Machinery gene
  VPBB_RS13385 (VPBB_2606) pilO 2911218..2911811 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  VPBB_RS13390 (VPBB_2607) pilN 2911804..2912382 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  VPBB_RS13395 (VPBB_2608) pilM 2912366..2913382 (-) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  VPBB_RS13400 (VPBB_2609) - 2913525..2916095 (+) 2571 WP_005480476.1 PBP1A family penicillin-binding protein -
  VPBB_RS13405 (VPBB_2610) oxyR 2916151..2917059 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=55507 VPBB_RS13390 WP_015297396.1 2911804..2912382(-) (pilN) [Vibrio parahaemolyticus BB22OP]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=55507 VPBB_RS13390 WP_015297396.1 2911804..2912382(-) (pilN) [Vibrio parahaemolyticus BB22OP]
GTGCTGTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGAGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCTCGGCTAGCAACCATGCTCGATAACATGGAGCGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTCGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTCAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594

Multiple sequence alignment