Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J7655_RS02780 Genome accession   NZ_CP072610
Coordinates   593696..595816 (+) Length   706 a.a.
NCBI ID   WP_230926465.1    Uniprot ID   -
Organism   Pseudomonas wenzhouensis strain A20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 588696..600816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7655_RS02760 (J7655_02750) pilM 590858..591922 (+) 1065 WP_037004588.1 pilus assembly protein PilM Machinery gene
  J7655_RS02765 (J7655_02755) - 591922..592497 (+) 576 WP_230926462.1 PilN domain-containing protein -
  J7655_RS02770 (J7655_02760) pilO 592494..593120 (+) 627 WP_230926463.1 type 4a pilus biogenesis protein PilO -
  J7655_RS02775 (J7655_02765) pilP 593117..593641 (+) 525 WP_230926464.1 type 4a pilus biogenesis lipoprotein PilP -
  J7655_RS02780 (J7655_02770) pilQ 593696..595816 (+) 2121 WP_230926465.1 type IV pilus secretin PilQ Machinery gene
  J7655_RS02785 (J7655_02775) aroK 595822..596340 (+) 519 WP_230926466.1 shikimate kinase AroK -
  J7655_RS02790 (J7655_02780) aroB 596392..597495 (+) 1104 WP_230926467.1 3-dehydroquinate synthase -
  J7655_RS02795 (J7655_02785) - 597506..599077 (+) 1572 WP_230926468.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 706 a.a.        Molecular weight: 76327.59 Da        Isoelectric Point: 4.8071

>NTDB_id=554557 J7655_RS02780 WP_230926465.1 593696..595816(+) (pilQ) [Pseudomonas wenzhouensis strain A20]
MNNSFSRLGLSVAALLMSPALLAASLNSVDVAALPGDRVELKLTFDEPVSEPRGYTIEQPARIALDLPGVSSRLGSKNRE
IGMGNARSLTVVEAKDRTRLIVNLTNLAPYTTRVDGNNLFVVVGDGPASTVSSVRASAPASPVTVVPAPTARAVTGVRSV
SDIDFQRGEQGEGNIVISLSDPKISPDVQEQGGKIRIDFPKTQLPESLRVRLDVKDFATPVQFVNASASADRASIVVEPT
GLYDYLVYQADNKLTISVKPLTEEDAVRRNADRFTYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVRGNITLRLQ
NVPWDQALDLVLKTKGLDQRKVGNVLMVAPADEIAAREQQELAVQKQVAELAPLRKDIIQLNYAKASDIAKLYEEGSSGT
LKDDMRGSVIFDDRTNSLIVTLTQEHIDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVRWGGNFWSGDKWNAWGK
NGNLGVEESDDGQQVGRFPGIRETPLNSPFVDLGVAGSTSGIGIGFVTNNVILDMQLSAMEKSGSGEVVSQPKVVTSDKE
TAKILKGTEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRVLMEVKVTKDAPDFSVASSTGGIPAISKNEVNAKVLV
ADGETIVIGGVYSNTQSKSVDKVPFLGDLPFIGRVFKRDLVQDRKAELLVFLTPRIMNNQAIAVNR

Nucleotide


Download         Length: 2121 bp        

>NTDB_id=554557 J7655_RS02780 WP_230926465.1 593696..595816(+) (pilQ) [Pseudomonas wenzhouensis strain A20]
ATGAACAACTCGTTCTCGCGGCTCGGTCTTTCTGTAGCGGCTCTTTTAATGTCGCCGGCGTTGTTGGCTGCTAGCCTAAA
TAGTGTGGATGTTGCCGCTTTGCCAGGTGATCGTGTCGAGTTGAAATTGACTTTTGATGAACCTGTTTCTGAGCCTCGAG
GTTATACCATTGAGCAGCCCGCTCGCATCGCTCTAGATTTACCGGGCGTATCCAGTCGTTTGGGTAGCAAGAATCGTGAA
ATAGGTATGGGTAATGCGCGCAGCCTCACTGTCGTTGAGGCGAAGGATCGCACACGGTTGATCGTCAACCTGACTAACCT
TGCGCCTTATACAACCCGCGTCGATGGCAATAATCTGTTCGTCGTCGTTGGTGACGGCCCTGCATCCACTGTTTCATCTG
TCAGGGCTTCTGCCCCGGCGAGTCCTGTTACCGTTGTTCCAGCACCCACTGCTCGCGCTGTTACAGGTGTTCGCTCGGTT
AGTGACATCGATTTTCAGCGTGGCGAGCAAGGGGAGGGTAATATCGTAATTTCTCTGTCCGACCCCAAAATCAGTCCAGA
TGTTCAAGAGCAGGGCGGGAAGATCCGTATTGATTTTCCCAAGACGCAGTTGCCCGAGTCGTTGCGCGTGCGTTTGGACG
TAAAGGATTTCGCTACACCAGTACAGTTCGTCAATGCCAGTGCTTCAGCTGATCGTGCAAGCATCGTGGTGGAACCTACC
GGACTTTATGACTACCTGGTGTACCAGGCAGACAACAAGCTGACCATCAGCGTCAAGCCTCTCACCGAAGAGGATGCGGT
TCGGCGTAACGCAGACCGTTTTACCTATACAGGTGAGAAACTCTCCCTGAACTTTCAGGACATTGATGTTCGTTCGGTCT
TGCAGTTGATCGCCGATTTTACGGATCTGAACCTGGTTGCCAGTGATACTGTTCGCGGCAATATCACTCTACGCCTCCAA
AACGTGCCGTGGGATCAGGCACTGGATCTGGTACTCAAGACCAAGGGGCTTGATCAGCGTAAGGTCGGTAATGTTTTGAT
GGTTGCTCCGGCTGATGAAATTGCCGCCCGTGAGCAGCAGGAGTTGGCTGTGCAGAAACAGGTTGCCGAGTTGGCGCCGC
TTCGCAAGGACATCATTCAGCTCAACTACGCGAAAGCATCCGACATCGCCAAGCTCTACGAGGAGGGTTCCTCGGGCACC
TTGAAGGACGATATGCGTGGCTCGGTAATTTTCGATGACCGCACCAACAGTCTGATCGTGACCCTGACTCAGGAGCATAT
CGACGAGCTCCGCCGTATTGTCACCCAACTGGATATCCCGGTTCGTCAGGTGATGATTGAAGCTCGCATTGTCGAAGCGA
ACGTCGACTATGACAAGGCTCTGGGGGTTCGCTGGGGGGGGAACTTCTGGTCGGGTGATAAGTGGAATGCTTGGGGCAAG
AACGGCAATCTCGGCGTTGAAGAGAGTGATGATGGGCAGCAGGTCGGTCGCTTCCCTGGCATCCGTGAAACTCCGCTGAA
TAGTCCTTTTGTTGATCTTGGTGTCGCTGGAAGCACTTCGGGTATTGGCATTGGCTTTGTTACCAATAACGTCATTCTCG
ATATGCAGCTCAGTGCCATGGAAAAATCCGGCTCTGGTGAGGTTGTATCTCAGCCTAAAGTTGTGACCTCCGACAAGGAA
ACCGCAAAGATCCTCAAAGGGACCGAAGTGCCTTATCAGGAAGCGAGCTCAAGTGGTGCAACCAGCACCTCTTTTAAGGA
GGCTGCGTTGTCGCTTGAGGTTACGCCGCAAATTACGCCAGACAACCGTGTGTTGATGGAGGTCAAAGTCACCAAGGATG
CTCCGGACTTCTCCGTCGCTTCGTCCACAGGCGGTATCCCGGCCATCAGCAAGAACGAGGTCAATGCCAAGGTTTTGGTT
GCTGATGGCGAGACCATCGTGATCGGTGGCGTGTATTCCAATACCCAGTCCAAGTCGGTGGATAAGGTGCCATTTTTGGG
TGATCTGCCCTTTATCGGTCGGGTGTTCAAGCGTGATCTGGTTCAGGATAGAAAGGCGGAATTGCTTGTATTCCTGACTC
CGCGCATCATGAACAACCAAGCCATTGCCGTGAACCGATGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(539-697)

Secretin and TonB N terminus short domain (STN)

(304-350)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-443)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.31

100

0.732
  comQ Acinetobacter baylyi ADP1

40.872

100

0.425
  pilQ Acinetobacter baumannii strain A118

39.09

100

0.414
  pilQ Acinetobacter baumannii D1279779

39.09

100

0.414