Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   J7655_RS02760 Genome accession   NZ_CP072610
Coordinates   590858..591922 (+) Length   354 a.a.
NCBI ID   WP_037004588.1    Uniprot ID   -
Organism   Pseudomonas wenzhouensis strain A20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 585858..596922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7655_RS02750 (J7655_02740) - 586904..588172 (+) 1269 WP_230926460.1 malic enzyme-like NAD(P)-binding protein -
  J7655_RS02755 (J7655_02745) - 588265..590673 (-) 2409 WP_420850928.1 penicillin-binding protein 1A -
  J7655_RS02760 (J7655_02750) pilM 590858..591922 (+) 1065 WP_037004588.1 pilus assembly protein PilM Machinery gene
  J7655_RS02765 (J7655_02755) - 591922..592497 (+) 576 WP_230926462.1 PilN domain-containing protein -
  J7655_RS02770 (J7655_02760) pilO 592494..593120 (+) 627 WP_230926463.1 type 4a pilus biogenesis protein PilO -
  J7655_RS02775 (J7655_02765) pilP 593117..593641 (+) 525 WP_230926464.1 type 4a pilus biogenesis lipoprotein PilP -
  J7655_RS02780 (J7655_02770) pilQ 593696..595816 (+) 2121 WP_230926465.1 type IV pilus secretin PilQ Machinery gene
  J7655_RS02785 (J7655_02775) aroK 595822..596340 (+) 519 WP_230926466.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37827.22 Da        Isoelectric Point: 4.4647

>NTDB_id=554556 J7655_RS02760 WP_037004588.1 590858..591922(+) (pilM) [Pseudomonas wenzhouensis strain A20]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLEAQLGAGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=554556 J7655_RS02760 WP_037004588.1 590858..591922(+) (pilM) [Pseudomonas wenzhouensis strain A20]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTATGCCGTCGAGCCACTTCCGCCCAATGCAGTGGTCGAGAAGAACA
TTGCCGAGTTGGAGGGGGTCGGTCAGGCGCTTTCTCGCGTTTTGGCCAAGGCCAAGAGCGGTGTCAAGACCGCGGCCGTT
GCCGTTGCCGGTTCGGCCGTCATCACCAAAACCATCGAAATGGAAGCTGGGCTTTCCGAGGATGATCTGGAAAACCAGCT
GAAGATCGAGGCGGATCAGTACATTCCCTATCCGCTGGAAGAAGTGGCCATCGATTTCGAGGTGCAGGGGCCGGCGCCGC
GTAATCCCGAGCGTGTCGAGGTTTTACTGGCGGCATGCCGCAAGGAAAACGTCGAGGTTCGTGAGGCTGCCCTGGCGCTC
GCAGGCCTGACTGCCAAAGTGGTTGACGTCGAGGCATACGCTCTCGAGCGTGCCTACGGTCTGCTGGAGGCGCAGCTGGG
TGCTGGTCACGACGAGTTGACGGTCGCGGTCGTGGATATCGGTGCGACCATGACTACGTTGAGCGTCCTGCACAACGGCC
GAACCATCTATACCCGCGAGCAACTCTTCGGCGGCAAGCAACTGACCGAGGAAATCCAACGTCGCTACGGTCTGTCCGTG
GAAGAAGCTGGACTTGCCAAGAAGCAAGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAGCCGTTCAAAGAAGC
TGTTGTTCAGCAGGTTTCCCGTTCGCTGCAGTTCTTCTTTGCTGCTGGGCAGTTCAACGATGTCGACTACATCCTCCTTG
CCGGCGGCACTGCATCGATTCCTGATCTCGATCGGTTGATCCAGCAAAAAATCGGTACGCAGACCCTGGTGGCTAATCCC
TTTGCCGATATGGCGCTCAGCAGCAAGGTCAATGCCGGTGCACTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.452