Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   J5X98_RS25475 Genome accession   NZ_CP072600
Coordinates   5905972..5907966 (+) Length   664 a.a.
NCBI ID   WP_223047796.1    Uniprot ID   -
Organism   Leptothermofonsia sichuanensis E412     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5900972..5912966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5X98_RS25455 (J5X98_25440) - 5900999..5901271 (-) 273 WP_223047792.1 sulfurtransferase TusA family protein -
  J5X98_RS25460 (J5X98_25445) dnaJ 5901268..5902398 (-) 1131 WP_223047793.1 molecular chaperone DnaJ -
  J5X98_RS25465 (J5X98_25450) dnaK 5902516..5904474 (-) 1959 WP_223047794.1 molecular chaperone DnaK -
  J5X98_RS25470 (J5X98_25455) grpE 5904669..5905394 (-) 726 WP_239033230.1 nucleotide exchange factor GrpE -
  J5X98_RS25475 (J5X98_25460) pilB/pilB1 5905972..5907966 (+) 1995 WP_223047796.1 GspE/PulE family protein Machinery gene
  J5X98_RS25480 (J5X98_25465) pilT 5908139..5909254 (+) 1116 WP_223047797.1 type IV pilus twitching motility protein PilT Machinery gene
  J5X98_RS25485 (J5X98_25470) - 5909405..5910628 (+) 1224 WP_223047798.1 type II secretion system F family protein -
  J5X98_RS25490 (J5X98_25475) - 5910633..5911256 (+) 624 WP_223047799.1 hypothetical protein -
  J5X98_RS25495 (J5X98_25480) - 5911346..5911498 (+) 153 WP_223047800.1 hypothetical protein -
  J5X98_RS25500 (J5X98_25485) - 5911648..5912604 (+) 957 WP_223047801.1 orange carotenoid-binding protein -

Sequence

Protein


Download         Length: 664 a.a.        Molecular weight: 74628.02 Da        Isoelectric Point: 4.9123

>NTDB_id=554511 J5X98_RS25475 WP_223047796.1 5905972..5907966(+) (pilB/pilB1) [Leptothermofonsia sichuanensis E412]
MTNSPSSRRALVVQNNFSPFGNKLIQSGYVNNEQMQQALIESRKTGRSLTDVLESITGQQLPPDLLRQYKKQQLFELKIL
YGVESLDPEISQISTSQVSELIDSLISIDLCRRYRLVPLSKNDETNSVLVAMVDPDNLGAQDDLNRILRPQQLSLQRLVI
TSEDYQRLINRYLDEQVERQRQKEQEEKVDVQADLEELGYLDLQDAPDEADANLDVADAEAAPVIALVNKIMIKALQEGV
SDIHIEPQEEYLRVRFRKDGVLRQAFDPFPKKIIPAVTARFKIIAQLDIAERRSPQDGRIRRIYDGRKIDFRVNTLPSRY
GEKVVLRILDNSATQLGLDKLITDPESLQIVQEMVRRPFGLILVTGPTGSGKTTTLYSALAERNDPGVNISTAEDPIEYT
LPGLTQVQVIREKGMDFAAILRAFLRQDPDVILVGETRDRETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVEPF
MVSGALIGVVAQRLVRRVCDECRIPYSPSREELARFGLSASRDGELTLYKANTLQPHEVQEARAKNELCPKCGGVGYKGR
CGVYEIMRVTERLQNMINQGAPTDSIKEVAVEEGMQTLLAYSLNLVRNGYTTLEEVERVTFTDTGLESELRAKRKSSLTC
KTCNADLKQEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 1995 bp        

>NTDB_id=554511 J5X98_RS25475 WP_223047796.1 5905972..5907966(+) (pilB/pilB1) [Leptothermofonsia sichuanensis E412]
ATGACTAACTCCCCATCGTCACGTCGCGCTCTTGTCGTACAAAACAATTTTTCTCCGTTCGGCAATAAGCTGATTCAGTC
TGGCTACGTCAACAATGAGCAGATGCAGCAGGCTCTGATTGAAAGTCGGAAGACTGGCAGGTCCTTGACTGATGTTCTGG
AGTCCATTACCGGCCAGCAGTTACCGCCTGATCTACTCCGCCAGTACAAAAAGCAACAGCTCTTTGAACTCAAAATTCTT
TACGGCGTAGAGTCCCTCGATCCGGAGATTAGTCAAATCTCGACCAGTCAGGTTAGTGAGCTGATTGACAGCCTGATTTC
AATCGACCTTTGCCGTCGCTACCGCCTGGTTCCATTGTCTAAGAACGATGAAACGAACAGTGTCTTAGTTGCAATGGTTG
ACCCAGACAATCTGGGTGCCCAGGATGACCTGAATCGGATTTTGCGCCCACAGCAGCTATCCTTACAACGGTTGGTAATC
ACCTCTGAAGATTATCAGCGGTTGATCAACCGCTACCTGGACGAACAGGTCGAGCGCCAGCGACAAAAAGAGCAGGAGGA
GAAAGTTGACGTTCAGGCGGATCTGGAAGAACTGGGCTATCTCGATTTGCAAGATGCCCCGGATGAAGCGGATGCCAATT
TAGATGTGGCGGATGCTGAAGCGGCTCCGGTTATTGCCCTGGTCAATAAAATTATGATCAAGGCTCTCCAGGAGGGGGTT
TCCGACATTCATATTGAACCTCAAGAGGAGTATCTGCGGGTTCGCTTCCGTAAAGATGGGGTGCTGCGGCAGGCCTTTGA
TCCATTTCCCAAAAAGATTATTCCGGCTGTGACCGCCCGCTTCAAAATTATTGCCCAACTGGATATTGCTGAACGTCGAT
CGCCTCAGGATGGGCGGATTCGTCGAATTTACGACGGACGCAAAATTGACTTCCGGGTTAACACGCTCCCCAGTCGTTAC
GGTGAGAAAGTGGTGCTGCGGATTCTGGATAACTCAGCAACCCAGTTGGGGCTGGATAAGTTGATTACAGATCCAGAATC
GCTCCAGATCGTTCAAGAAATGGTGCGGCGACCCTTTGGGTTGATTCTGGTAACAGGTCCCACGGGTTCTGGTAAAACGA
CAACGCTCTATTCTGCTCTGGCTGAGAGAAATGACCCTGGGGTCAACATCAGCACGGCGGAAGACCCGATTGAATATACC
CTACCGGGGCTGACTCAGGTGCAGGTAATCCGTGAGAAGGGGATGGACTTTGCCGCCATCTTGAGAGCTTTCCTGCGGCA
AGACCCGGATGTGATTCTGGTGGGTGAAACACGCGACCGGGAAACGGCAAAAACGGCAATTGAGGCAGCACTTACCGGAC
ACCTGGTTTTGACAACCCTGCATACCAATGACGCGGCTGGCGCGATCGCCCGTCTGGATGAAATGGGGGTGGAACCTTTC
ATGGTTTCTGGGGCACTGATTGGTGTCGTTGCCCAGCGCCTGGTGCGGCGAGTCTGCGATGAATGCCGCATCCCTTACAG
TCCCTCCAGAGAAGAACTGGCCAGATTTGGTCTGTCCGCATCGAGAGATGGCGAACTTACCCTTTACAAAGCCAATACTC
TCCAACCTCATGAGGTTCAGGAAGCCCGCGCCAAAAACGAGCTTTGCCCTAAATGTGGCGGTGTTGGGTATAAGGGACGT
TGCGGGGTGTACGAAATCATGCGTGTGACTGAGCGCCTGCAAAACATGATTAACCAGGGTGCTCCCACCGACAGCATCAA
AGAGGTGGCGGTCGAAGAAGGCATGCAAACCCTGCTGGCTTATAGCCTCAACCTGGTTCGGAATGGTTATACTACGCTGG
AAGAGGTTGAGCGTGTGACCTTTACGGATACTGGACTGGAATCTGAACTGAGAGCCAAGCGCAAATCCTCTCTCACCTGC
AAAACCTGTAATGCAGACCTCAAACAGGAATGGCTGGACTGCCCCTACTGTATGACACCCCGCTTCCAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-177)

Type II/IV secretion system protein (T2SSE)

(230-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

69.289

99.548

0.69
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.005

92.922

0.381
  pilF Thermus thermophilus HB27

39.355

93.373

0.367