Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J7445_RS11650 Genome accession   NZ_CP072524
Coordinates   2475485..2477620 (+) Length   711 a.a.
NCBI ID   WP_070655368.1    Uniprot ID   -
Organism   Neisseria sicca strain NS20201025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2478058..2494555 2475485..2477620 flank 438


Gene organization within MGE regions


Location: 2475485..2494555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7445_RS11650 (J7445_11650) pilQ 2475485..2477620 (+) 2136 WP_070655368.1 type IV pilus secretin PilQ Machinery gene
  J7445_RS11655 (J7445_11655) - 2478058..2478594 (+) 537 WP_244969483.1 shikimate kinase -
  J7445_RS11660 (J7445_11660) - 2478668..2479159 (+) 492 Protein_2257 helix-turn-helix domain-containing protein -
  J7445_RS11670 (J7445_11670) - 2479306..2480313 (-) 1008 WP_209283070.1 IS5 family transposase -
  J7445_RS11680 (J7445_11680) - 2480618..2480977 (-) 360 WP_003743735.1 roadblock/LC7 domain-containing protein -
  J7445_RS11685 (J7445_11685) - 2481017..2481658 (-) 642 WP_070654376.1 peptidase M23 -
  J7445_RS11690 (J7445_11690) - 2481664..2482200 (-) 537 WP_003743737.1 ATP/GTP-binding protein -
  J7445_RS11695 (J7445_11695) - 2482213..2483307 (-) 1095 WP_070654375.1 response regulator -
  J7445_RS11700 (J7445_11700) - 2483332..2484783 (-) 1452 WP_070654374.1 23S rRNA methyltransferase -
  J7445_RS11705 (J7445_11705) rlmD 2484929..2486275 (-) 1347 WP_244969485.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  J7445_RS11710 (J7445_11710) - 2486402..2487400 (+) 999 WP_209283071.1 L,D-transpeptidase -
  J7445_RS11715 (J7445_11715) - 2487542..2487652 (+) 111 Protein_2266 IS5/IS1182 family transposase -
  J7445_RS11720 (J7445_11720) - 2487827..2488012 (-) 186 Protein_2267 transposase -
  J7445_RS11725 (J7445_11725) - 2488180..2488509 (+) 330 Protein_2268 transposase -
  J7445_RS11730 (J7445_11730) - 2488573..2489580 (+) 1008 WP_209283352.1 IS5 family transposase -
  J7445_RS11735 (J7445_11735) - 2489823..2491511 (-) 1689 WP_045075230.1 cation acetate symporter -
  J7445_RS11740 (J7445_11740) - 2491622..2491984 (-) 363 WP_070655303.1 DUF485 domain-containing protein -
  J7445_RS11745 (J7445_11745) - 2492341..2493237 (+) 897 WP_070655305.1 LysR family transcriptional regulator -
  J7445_RS11750 (J7445_11750) - 2493277..2493985 (-) 709 Protein_2273 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -
  J7445_RS11755 (J7445_11755) - 2494034..2494447 (+) 414 Protein_2274 EamA family transporter -

Sequence

Protein


Download         Length: 711 a.a.        Molecular weight: 77678.90 Da        Isoelectric Point: 9.7158

>NTDB_id=553928 J7445_RS11650 WP_070655368.1 2475485..2477620(+) (pilQ) [Neisseria sicca strain NS20201025]
MKTKNMTKLFAGISMIAAAQIALAGNITDINVSTLPDSQKIIKIRFDRDVISPSGFVTSTPSRIALDFAKTNIQLAQPVL
EYADPLLNQITAAQNNDRSRIVLGLNKAAQYNTEVHGNEVWVFVTESSDRTETSTAISNNANARQKASSSETVKQTVNSA
NIDFRKGAHNSGIVELNAPAFTGKPEVKQQRDRVIITLKNYPLPTQAQRSLDVADFSTPVKNITLKRIGNDAQLIIRNNG
NWDFNTKAANGQFAFEVMPKSASTESSGLNANPNKSFRGRKISLDFQDIEVRTILQILAKESGVNIVASDSVNGKMTLSL
KDVPWDQALDLVMQARNLDMRRQGNIINIAPRDELLAKDKAFLQAEKEIAELGPLYSQTFQLKYKNVEEFRKILRLDDNN
SGNSNGRNTLLSGRGSALIDPATNTLIITDNRNVVEKFRKLIEELDVPTRQVMVEARIVEAKDGFSRDLGVKFGVAGSKG
RNAWGNSWSNAQDNHNTNSAVLRGDANYRTWSLQPNISLPAAAATNNIALVRALSSGALGLEISASEATGKSKTISNPRV
LTQDRREATIESGTEIPYQEASSSGATSVSFKKAVLGLTVTPNVTPDGQVIMTVKITKDTPQDCTVDSLITKCIQTKNLN
TQAMVEDGGTLIVGGIYEEDNSDTINKVPLLGDLPVVGNLFKSRAKKEERRELLVFITPRIMDGVGSNLRY

Nucleotide


Download         Length: 2136 bp        

>NTDB_id=553928 J7445_RS11650 WP_070655368.1 2475485..2477620(+) (pilQ) [Neisseria sicca strain NS20201025]
ATGAAAACTAAGAATATGACGAAATTATTTGCCGGCATCAGTATGATTGCGGCAGCACAAATTGCGTTGGCAGGTAATAT
TACTGATATTAATGTATCAACTTTGCCGGATAGTCAAAAAATTATCAAAATACGTTTTGACCGGGATGTGATTAGCCCGA
GCGGCTTTGTTACATCCACCCCGTCCCGAATTGCCTTGGATTTTGCCAAAACAAATATTCAATTGGCTCAACCTGTATTG
GAATACGCAGATCCTTTGTTAAATCAAATTACTGCGGCTCAGAATAATGACCGATCTCGCATTGTTTTGGGATTGAATAA
AGCAGCGCAATATAATACCGAAGTCCATGGTAATGAAGTATGGGTATTCGTTACAGAATCATCTGATCGAACCGAAACTT
CTACGGCTATTTCAAATAATGCAAATGCACGTCAGAAGGCAAGCTCGTCTGAAACAGTTAAACAAACGGTAAATTCAGCT
AATATTGACTTCAGGAAAGGTGCACATAACTCAGGTATTGTGGAATTGAATGCGCCTGCTTTCACAGGCAAACCTGAAGT
GAAGCAACAACGTGATCGGGTGATTATTACTCTGAAAAATTATCCGTTGCCAACACAAGCGCAACGCAGTTTGGATGTTG
CAGATTTCAGTACACCGGTTAAAAATATTACGTTAAAACGTATTGGAAATGATGCCCAACTCATTATTCGCAATAATGGC
AATTGGGATTTCAATACCAAAGCTGCAAATGGTCAATTTGCTTTTGAAGTAATGCCTAAGTCCGCTAGTACGGAATCAAG
TGGGTTGAATGCAAATCCAAACAAATCTTTCCGCGGCCGTAAAATTTCCCTGGATTTTCAAGATATTGAGGTGCGTACCA
TTTTGCAAATCCTTGCGAAGGAGTCCGGGGTTAATATCGTGGCCAGTGATTCCGTGAATGGCAAAATGACACTTTCTTTG
AAAGATGTGCCTTGGGATCAGGCTTTGGATTTGGTTATGCAGGCGCGTAATTTGGATATGCGCCGACAAGGAAATATCAT
CAATATTGCACCTCGTGATGAATTGTTGGCCAAAGATAAGGCTTTCTTACAGGCAGAGAAAGAAATTGCCGAATTGGGGC
CTTTATATTCCCAAACCTTCCAATTAAAATACAAGAATGTAGAAGAGTTTCGTAAAATTTTACGTTTGGACGACAACAAT
AGTGGAAACTCCAATGGTAGAAATACCTTGTTGAGTGGCCGTGGTAGTGCGCTGATTGATCCTGCAACGAATACTTTGAT
TATTACAGATAATCGAAATGTTGTAGAAAAATTCCGTAAGTTGATTGAGGAGTTGGATGTACCGACTCGTCAAGTCATGG
TTGAAGCCCGTATTGTTGAAGCAAAAGACGGTTTTTCACGTGATTTGGGGGTGAAATTCGGTGTCGCAGGTTCCAAAGGA
CGCAACGCTTGGGGTAACTCTTGGAGTAATGCGCAAGATAACCATAATACTAATTCAGCTGTTTTGCGAGGTGATGCTAA
TTACCGTACATGGTCTTTACAACCGAATATTAGCCTGCCTGCAGCAGCAGCGACTAATAATATTGCTCTAGTACGAGCTT
TGTCATCAGGTGCATTGGGTTTGGAAATTTCTGCTTCGGAAGCTACTGGTAAAAGTAAGACCATTTCTAATCCGCGTGTC
CTGACTCAAGACCGTAGAGAGGCTACTATTGAGTCCGGTACTGAAATTCCGTATCAGGAGGCTTCTTCCAGTGGTGCAAC
TTCGGTTTCGTTCAAAAAAGCCGTGTTGGGCTTAACGGTAACGCCGAATGTAACGCCTGATGGTCAAGTTATCATGACTG
TAAAAATTACCAAAGATACGCCGCAAGACTGTACAGTGGACTCTTTGATTACCAAATGTATTCAGACTAAAAACCTGAAT
ACTCAGGCAATGGTTGAAGATGGTGGCACGTTGATTGTTGGCGGTATCTATGAAGAAGATAATAGCGATACGATTAACAA
AGTACCATTATTGGGCGATTTGCCAGTGGTAGGCAATTTGTTCAAATCCCGTGCGAAAAAAGAAGAGCGTCGTGAATTGC
TGGTTTTCATCACTCCGCGAATTATGGATGGTGTAGGCAGCAATCTCCGCTACTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(546-703)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-451)

AMIN domain (AMIN)

(41-120)

Secretin and TonB N terminus short domain (STN)

(304-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

61.892

100

0.644
  pilQ Neisseria meningitidis MC58

66.726

78.622

0.525
  pilQ Neisseria meningitidis M1080

66.726

78.622

0.525