Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   B938_RS12255 Genome accession   NC_019842
Coordinates   2565895..2566509 (-) Length   204 a.a.
NCBI ID   WP_015240245.1    Uniprot ID   -
Organism   Bacillus velezensis AS43.3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2560895..2571509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B938_RS12230 (B938_12315) rpsT 2561199..2561465 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  B938_RS12235 (B938_12320) holA 2561481..2562522 (-) 1042 Protein_2414 DNA polymerase III subunit delta -
  B938_RS20145 - 2562562..2562713 (-) 152 Protein_2415 hypothetical protein -
  B938_RS19410 (B938_12325) - 2562754..2562888 (+) 135 WP_003152870.1 YqzM family protein -
  B938_RS12245 (B938_12330) comEC 2562907..2565258 (-) 2352 WP_015240244.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  B938_RS12250 (B938_12335) - 2565259..2565828 (-) 570 WP_003152868.1 ComE operon protein 2 -
  B938_RS12255 (B938_12340) comEA 2565895..2566509 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  B938_RS12260 (B938_12345) comER 2566568..2567389 (+) 822 WP_015240246.1 late competence protein ComER -
  B938_RS12265 (B938_12350) - 2567458..2568195 (-) 738 WP_015240247.1 class I SAM-dependent DNA methyltransferase -
  B938_RS12270 (B938_12355) rsfS 2568192..2568548 (-) 357 WP_007408260.1 ribosome silencing factor -
  B938_RS12275 (B938_12360) yqeK 2568566..2569126 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  B938_RS12280 (B938_12365) - 2569116..2569685 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  B938_RS12285 (B938_12370) yhbY 2569696..2569986 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  B938_RS12290 (B938_12375) aroE 2569980..2570822 (-) 843 WP_015240248.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22209.32 Da        Isoelectric Point: 5.5211

>NTDB_id=55163 B938_RS12255 WP_015240245.1 2565895..2566509(-) (comEA) [Bacillus velezensis AS43.3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=55163 B938_RS12255 WP_015240245.1 2565895..2566509(-) (comEA) [Bacillus velezensis AS43.3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment