Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J5X95_RS01640 Genome accession   NZ_CP072120
Coordinates   328917..329987 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus amyloliquefaciens strain GKT04     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323917..334987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5X95_RS01590 (J5X95_01590) sipW 323977..324561 (-) 585 WP_015240205.1 signal peptidase I SipW -
  J5X95_RS01595 (J5X95_01595) tapA 324533..325204 (-) 672 WP_124934997.1 amyloid fiber anchoring/assembly protein TapA -
  J5X95_RS01600 (J5X95_01600) - 325463..325792 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  J5X95_RS01605 (J5X95_01605) - 325832..326011 (-) 180 WP_003153093.1 YqzE family protein -
  J5X95_RS01610 (J5X95_01610) comGG 326068..326445 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  J5X95_RS01615 (J5X95_01615) comGF 326446..326946 (-) 501 WP_257899725.1 competence type IV pilus minor pilin ComGF -
  J5X95_RS01620 (J5X95_01620) comGE 326855..327169 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  J5X95_RS01625 (J5X95_01625) comGD 327153..327590 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  J5X95_RS01630 (J5X95_01630) comGC 327580..327888 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  J5X95_RS01635 (J5X95_01635) comGB 327893..328930 (-) 1038 WP_094031834.1 competence type IV pilus assembly protein ComGB Machinery gene
  J5X95_RS01640 (J5X95_01640) comGA 328917..329987 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  J5X95_RS01645 (J5X95_01645) - 330179..331129 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  J5X95_RS01650 (J5X95_01650) - 331275..332576 (+) 1302 WP_039063318.1 hemolysin family protein -
  J5X95_RS01655 (J5X95_01655) - 332614..333444 (-) 831 WP_007408317.1 STAS domain-containing protein -
  J5X95_RS01665 (J5X95_01665) - 333651..334031 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  J5X95_RS01670 (J5X95_01670) - 334269..334514 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=550684 J5X95_RS01640 WP_012117985.1 328917..329987(-) (comGA) [Bacillus amyloliquefaciens strain GKT04]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=550684 J5X95_RS01640 WP_012117985.1 328917..329987(-) (comGA) [Bacillus amyloliquefaciens strain GKT04]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374