Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   J6L63_RS02400 Genome accession   NZ_CP072116
Coordinates   447079..448149 (+) Length   356 a.a.
NCBI ID   WP_000776422.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TCH32929     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442079..453149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J6L63_RS02370 (J6L63_02370) - 442502..443965 (+) 1464 WP_001017852.1 rhomboid family intramembrane serine protease -
  J6L63_RS02375 (J6L63_02375) - 443946..444149 (+) 204 WP_000087561.1 YqgQ family protein -
  J6L63_RS02380 (J6L63_02380) - 444146..445132 (+) 987 WP_000161311.1 ROK family glucokinase -
  J6L63_RS02385 (J6L63_02385) - 445132..445461 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  J6L63_RS02390 (J6L63_02390) - 445458..446081 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  J6L63_RS02395 (J6L63_02395) comGA 446133..447107 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  J6L63_RS02400 (J6L63_02400) comGB 447079..448149 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  J6L63_RS02405 (J6L63_02405) comGC 448163..448474 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  J6L63_RS02410 (J6L63_02410) comGD 448452..448898 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  J6L63_RS02415 (J6L63_02415) comGE 448885..449184 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  J6L63_RS02420 (J6L63_02420) comGF 449102..449599 (+) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  J6L63_RS02425 (J6L63_02425) - 449696..449842 (+) 147 WP_001792109.1 hypothetical protein -
  J6L63_RS02430 (J6L63_02430) - 449832..450356 (+) 525 WP_001015120.1 shikimate kinase -
  J6L63_RS02435 (J6L63_02435) gcvT 450515..451606 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  J6L63_RS02440 (J6L63_02440) gcvPA 451626..452972 (+) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41447.84 Da        Isoelectric Point: 10.0606

>NTDB_id=550576 J6L63_RS02400 WP_000776422.1 447079..448149(+) (comGB) [Staphylococcus aureus strain TCH32929]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=550576 J6L63_RS02400 WP_000776422.1 447079..448149(+) (comGB) [Staphylococcus aureus strain TCH32929]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

100

100

1
  comGB Staphylococcus aureus N315

100

100

1