Detailed information NTG0549505

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: J4048_RS13180
• Genome accession: NZ_CP071970
• Coordinates: 2717827..2718441 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain XJ5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2712827..2723441

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J4048_RS13150 (J4048_13150)	rpsT	2713130..2713396 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
J4048_RS13155 (J4048_13155)	holA	2713413..2714454 (-)	1042	Protein_2571	DNA polymerase III subunit delta	-
J4048_RS13160 (J4048_13160)	-	2714494..2714645 (-)	152	Protein_2572	hypothetical protein	-
J4048_RS13165 (J4048_13165)	-	2714686..2714820 (+)	135	WP_003152870.1	YqzM family protein	-
J4048_RS13170 (J4048_13170)	comEC	2714839..2717190 (-)	2352	WP_039254415.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
J4048_RS13175 (J4048_13175)	-	2717191..2717760 (-)	570	WP_003152868.1	ComE operon protein 2	-
J4048_RS13180 (J4048_13180)	comEA	2717827..2718441 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
J4048_RS13185 (J4048_13185)	comER	2718500..2719321 (+)	822	WP_039254409.1	late competence protein ComER	-
J4048_RS13190 (J4048_13190)	-	2719390..2720127 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
J4048_RS13195 (J4048_13195)	rsfS	2720124..2720480 (-)	357	WP_007408260.1	ribosome silencing factor	-
J4048_RS13200 (J4048_13200)	yqeK	2720498..2721058 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
J4048_RS13205 (J4048_13205)	-	2721048..2721617 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
J4048_RS13210 (J4048_13210)	yhbY	2721628..2721918 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
J4048_RS13215 (J4048_13215)	aroE	2721912..2722754 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=549505 J4048_RS13180 WP_015240245.1 2717827..2718441(-) (comEA) [Bacillus amyloliquefaciens strain XJ5]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=549505 J4048_RS13180 WP_015240245.1 2717827..2718441(-) (comEA) [Bacillus amyloliquefaciens strain XJ5]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363