Detailed information NTG0548719

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LSP21_RS01295
• Genome accession: NZ_CP089068
• Coordinates: 285991..286863 (+)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 280991..291863

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSP21_RS01280 (LSP21_01280)	pilA	282374..282838 (-)	465	WP_003122079.1	pilin	Machinery gene
LSP21_RS01285 (LSP21_01285)	pilB	283069..284769 (+)	1701	WP_003107297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LSP21_RS01290 (LSP21_01290)	pilC	284773..285990 (+)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
LSP21_RS01295 (LSP21_01295)	pilD	285991..286863 (+)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
LSP21_RS01300 (LSP21_01300)	coaE	286860..287471 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
LSP21_RS01305 (LSP21_01305)	yacG	287468..287668 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
LSP21_RS01310 (LSP21_01310)	-	287705..287914 (-)	210	WP_017002071.1	hypothetical protein	-
LSP21_RS01315 (LSP21_01315)	-	288020..288709 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
LSP21_RS01320 (LSP21_01320)	-	288706..289176 (-)	471	WP_003103866.1	hypothetical protein	-
LSP21_RS01325 (LSP21_01325)	-	289173..289598 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
LSP21_RS01330 (LSP21_01330)	-	289731..290360 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
LSP21_RS01335 (LSP21_01335)	-	290357..290806 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
LSP21_RS01340 (LSP21_01340)	-	290832..291002 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=548719 LSP21_RS01295 WP_003107301.1 285991..286863(+) (pilD) [Pseudomonas aeruginosa strain UNC_PaerCF13]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=548719 LSP21_RS01295 WP_003107301.1 285991..286863(+) (pilD) [Pseudomonas aeruginosa strain UNC_PaerCF13]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGTGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469