Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J4N39_RS01200 Genome accession   NZ_CP071848
Coordinates   250280..250864 (+) Length   194 a.a.
NCBI ID   WP_252021232.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43136     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 245280..255864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N39_RS01185 (J4N39_01185) - 246068..248629 (-) 2562 WP_252021226.1 PBP1A family penicillin-binding protein -
  J4N39_RS01190 (J4N39_01190) pilM 248774..249730 (+) 957 WP_252021228.1 pilus assembly protein PilM -
  J4N39_RS01195 (J4N39_01195) - 249717..250277 (+) 561 WP_252021230.1 PilN domain-containing protein -
  J4N39_RS01200 (J4N39_01200) pilO 250280..250864 (+) 585 WP_252021232.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N39_RS01205 (J4N39_01205) - 250851..251372 (+) 522 WP_252021234.1 pilus assembly protein PilP -
  J4N39_RS01210 (J4N39_01210) pilQ 251411..252826 (+) 1416 WP_252021236.1 type IV pilus secretin PilQ Machinery gene
  J4N39_RS01215 (J4N39_01215) aroK 253068..253586 (+) 519 WP_252021238.1 shikimate kinase AroK -
  J4N39_RS01220 (J4N39_01220) aroB 253612..254697 (+) 1086 WP_252021240.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22843.00 Da        Isoelectric Point: 5.3094

>NTDB_id=548274 J4N39_RS01200 WP_252021232.1 250280..250864(+) (pilO) [Vibrio sp. SCSIO 43136]
MQSLEMDEFAYWPRVYQWGVIALVCFALLLLGWWFYLVPQHQALSHEEQQEQSLKSLFEIKARQVAALPKYDQQLKLLNQ
RYQLLQQQLPEQKELATLLASINELGIKHDLTFTRIDWGERQVTSELYRYPLNIELTGSYENIGDFSQAIGELPRIVNFA
DVDWQRVSQESKTLHFRVRAFAYQFRPGSTDAEK

Nucleotide


Download         Length: 585 bp        

>NTDB_id=548274 J4N39_RS01200 WP_252021232.1 250280..250864(+) (pilO) [Vibrio sp. SCSIO 43136]
ATGCAGTCATTAGAGATGGATGAATTTGCCTATTGGCCACGAGTTTATCAATGGGGCGTGATTGCTTTGGTGTGCTTTGC
ACTTCTGCTGTTAGGTTGGTGGTTTTACTTAGTGCCACAGCATCAAGCGCTTAGCCATGAAGAGCAGCAAGAACAAAGTC
TTAAATCGTTGTTTGAAATTAAAGCTCGTCAAGTGGCCGCGTTGCCTAAGTATGACCAGCAGCTCAAGCTATTGAATCAA
CGTTACCAGCTGTTGCAACAACAGTTGCCCGAACAAAAAGAGCTAGCGACTTTGCTTGCTTCGATAAATGAGCTTGGTAT
TAAGCACGATCTCACTTTTACCCGTATTGATTGGGGAGAGCGCCAAGTGACTAGCGAGCTCTATCGCTACCCATTGAATA
TTGAACTGACAGGCAGCTACGAGAATATCGGAGATTTCAGTCAGGCCATTGGCGAGCTGCCAAGGATAGTTAACTTTGCT
GACGTAGATTGGCAGCGGGTAAGCCAAGAAAGCAAAACCTTGCACTTTCGTGTTCGAGCCTTTGCCTATCAGTTTAGACC
GGGGTCCACAGATGCAGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(44-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

59.677

95.876

0.572
  pilO Vibrio cholerae strain A1552

56.545

98.454

0.557