Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J4N43_RS01610 Genome accession   NZ_CP071842
Coordinates   341552..342145 (+) Length   197 a.a.
NCBI ID   WP_053350733.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43009     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 336552..347145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N43_RS01595 (J4N43_01595) - 337278..339839 (-) 2562 WP_005381325.1 PBP1A family penicillin-binding protein -
  J4N43_RS01600 (J4N43_01600) pilM 339984..341000 (+) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene
  J4N43_RS01605 (J4N43_01605) pilN 340984..341559 (+) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  J4N43_RS01610 (J4N43_01610) pilO 341552..342145 (+) 594 WP_053350733.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N43_RS01615 (J4N43_01615) pilP 342135..342650 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  J4N43_RS01620 (J4N43_01620) pilQ 342676..344430 (+) 1755 WP_252094125.1 type IV pilus secretin PilQ family protein Machinery gene
  J4N43_RS01625 (J4N43_01625) aroK 344618..345136 (+) 519 WP_005381319.1 shikimate kinase AroK -
  J4N43_RS01630 (J4N43_01630) aroB 345169..346269 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23029.56 Da        Isoelectric Point: 5.0948

>NTDB_id=548188 J4N43_RS01610 WP_053350733.1 341552..342145(+) (pilO) [Vibrio sp. SCSIO 43009]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMVIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=548188 J4N43_RS01610 WP_053350733.1 341552..342145(+) (pilO) [Vibrio sp. SCSIO 43009]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAAATTGTGGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGTCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGTGCTAATACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685