Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J4N43_RS01620 Genome accession   NZ_CP071842
Coordinates   342676..344430 (+) Length   584 a.a.
NCBI ID   WP_252094125.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43009     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337676..349430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N43_RS01600 (J4N43_01600) pilM 339984..341000 (+) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene
  J4N43_RS01605 (J4N43_01605) pilN 340984..341559 (+) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  J4N43_RS01610 (J4N43_01610) pilO 341552..342145 (+) 594 WP_053350733.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N43_RS01615 (J4N43_01615) pilP 342135..342650 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  J4N43_RS01620 (J4N43_01620) pilQ 342676..344430 (+) 1755 WP_252094125.1 type IV pilus secretin PilQ family protein Machinery gene
  J4N43_RS01625 (J4N43_01625) aroK 344618..345136 (+) 519 WP_005381319.1 shikimate kinase AroK -
  J4N43_RS01630 (J4N43_01630) aroB 345169..346269 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -
  J4N43_RS01635 (J4N43_01635) - 346322..347833 (+) 1512 WP_025768079.1 AAA family ATPase -
  J4N43_RS01640 (J4N43_01640) - 347903..348742 (+) 840 WP_005382762.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63868.84 Da        Isoelectric Point: 4.4593

>NTDB_id=548190 J4N43_RS01620 WP_252094125.1 342676..344430(+) (pilQ) [Vibrio sp. SCSIO 43009]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKASDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGGDGGDGGDGDGVGIDDFLNVNLAATSP
NATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVT
PQITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGAL
FRRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=548190 J4N43_RS01620 WP_252094125.1 342676..344430(+) (pilQ) [Vibrio sp. SCSIO 43009]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGATTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCTCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGCTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTTACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGTGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGTGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
GTGACGGTGGTGACGGTGGTGACGGTGATGGAGTAGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGCCCG
AATGCGACCAGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCGCTGCAACA
AGAGTCTAAAGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCTGCCTATATCGAACAAGGTACAG
AGATTCCTTACCTAGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTAACC
CCGCAAATCACACCGGATAACCGCTTAGTATTGGATTTAAGTGTCACGCAAGATAGACCAGGACAAGTCGTGAAGACAGG
CACTGGTGAAGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTG
GTGGTATTTTTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGACCTGCCGCTATTAGGTGCTCTT
TTTCGTCGAAGTTATGAGAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(424-582)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.466

100

0.925
  pilQ Vibrio cholerae strain A1552

74.359

100

0.745
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.359

100

0.745
  pilQ Pseudomonas aeruginosa PAK

39.931

98.63

0.394
  pilQ Legionella pneumophila strain ERS1305867

40.497

96.404

0.39
  pilQ Legionella pneumophila strain Lp02

40.497

96.404

0.39
  comQ Acinetobacter baylyi ADP1

37.285

99.658

0.372
  pilQ Acinetobacter baumannii strain A118

35.871

100

0.363
  pilQ Acinetobacter baumannii D1279779

35.871

100

0.363