Detailed information    

experimental Experimentally validated

Overview

Name   pilZ   Type   Machinery gene
Locus tag   GCO85_RS07110 Genome accession   NZ_CP048618
Coordinates   1562531..1562872 (+) Length   113 a.a.
NCBI ID   WP_025519545.1    Uniprot ID   A0A128V7G5
Organism   Legionella pneumophila strain ERS1305867     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The ΔpilZ mutant is defective for DNA uptake and is totally unable to produce extracellular PilA2 or assemble PilA2 filaments.


Genomic Context

Location: 1557531..1567872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS07085 (GCO85_07095) acpP 1558360..1558608 (+) 249 WP_003631752.1 acyl carrier protein -
  GCO85_RS07090 (GCO85_07100) fabF 1558628..1559866 (+) 1239 WP_011213689.1 beta-ketoacyl-ACP synthase II -
  GCO85_RS07095 (GCO85_07105) mltG 1559867..1560865 (+) 999 WP_011213690.1 endolytic transglycosylase MltG -
  GCO85_RS07100 (GCO85_07110) tmk 1560865..1561503 (+) 639 WP_027226597.1 dTMP kinase -
  GCO85_RS07105 (GCO85_07115) - 1561500..1562405 (+) 906 WP_027226596.1 DNA polymerase III subunit delta' -
  GCO85_RS07110 (GCO85_07120) pilZ 1562531..1562872 (+) 342 WP_025519545.1 PilZ domain-containing protein Machinery gene
  GCO85_RS07115 (GCO85_07125) - 1562932..1563720 (+) 789 WP_011213694.1 TatD family hydrolase -
  GCO85_RS07120 (GCO85_07130) - 1563832..1564932 (+) 1101 WP_061636333.1 anhydro-N-acetylmuramic acid kinase -
  GCO85_RS07125 (GCO85_07135) - 1564988..1566280 (+) 1293 WP_011213696.1 MFS transporter -

Sequence

Protein


Download         Length: 113 a.a.        Molecular weight: 12521.54 Da        Isoelectric Point: 8.2421

>NTDB_id=1261 GCO85_RS07110 WP_025519545.1 1562531..1562872(+) (pilZ) [Legionella pneumophila strain ERS1305867]
MTEKPQLINCSFPNEASLYMAYMPFVKGGGLFIRTQHVYSLGQSLELSVKLLNENEPYLVTGKIVWITPKGAQGNKPPGV
GIQFTGENSRYLCNKIETYLAGMLKSCQVTDTI

Nucleotide


Download         Length: 342 bp        

>NTDB_id=1261 GCO85_RS07110 WP_025519545.1 1562531..1562872(+) (pilZ) [Legionella pneumophila strain ERS1305867]
ATGACCGAAAAACCACAGCTTATAAACTGCTCATTTCCCAATGAAGCATCTTTGTACATGGCTTACATGCCTTTTGTGAA
GGGTGGCGGTCTTTTTATAAGAACTCAACACGTTTATTCTCTAGGGCAGTCGCTTGAATTATCTGTTAAATTGCTTAATG
AAAATGAACCATATTTGGTCACTGGTAAAATAGTCTGGATTACTCCTAAAGGTGCACAAGGTAATAAGCCGCCGGGAGTT
GGTATTCAATTTACCGGTGAAAACAGCCGTTATCTATGTAACAAGATAGAAACTTATTTGGCAGGAATGCTAAAATCATG
TCAGGTTACAGATACTATCTAG

Domains

Predicted by InterproScan.

PilZ domain (PilZ)

(8-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A128V7G5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilZ Legionella pneumophila str. Paris

100

100

1

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]