Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J4G45_RS13760 Genome accession   NZ_CP071770
Coordinates   2432231..2433091 (+) Length   286 a.a.
NCBI ID   WP_004975862.1    Uniprot ID   A0A4R0DGY7
Organism   Acinetobacter towneri strain GX5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2427231..2438091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4G45_RS12700 (J4G45_12705) secG 2427850..2428179 (-) 330 WP_004975868.1 preprotein translocase subunit SecG -
  J4G45_RS12705 (J4G45_12710) tpiA 2428193..2428984 (-) 792 WP_019836081.1 triose-phosphate isomerase -
  J4G45_RS12710 (J4G45_12715) pilB 2429262..2430974 (+) 1713 WP_179996326.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4G45_RS12715 (J4G45_12720) pilC 2431005..2432231 (+) 1227 WP_004975864.1 type II secretion system F family protein Machinery gene
  J4G45_RS13760 (J4G45_12725) pilD 2432231..2433091 (+) 861 WP_004975862.1 prepilin peptidase Machinery gene
  J4G45_RS12725 (J4G45_12730) coaE 2433101..2433700 (+) 600 WP_004975860.1 dephospho-CoA kinase -
  J4G45_RS12730 (J4G45_12735) - 2433706..2434584 (-) 879 WP_042068576.1 DMT family transporter -
  J4G45_RS12735 (J4G45_12740) rlmB 2434679..2435428 (-) 750 WP_004975856.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  J4G45_RS12740 (J4G45_12745) - 2435617..2435940 (-) 324 WP_004975854.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32041.41 Da        Isoelectric Point: 8.0564

>NTDB_id=547652 J4G45_RS13760 WP_004975862.1 2432231..2433091(+) (pilD) [Acinetobacter towneri strain GX5]
MQELIAYFIQNPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIIDESKITLSKPASTCPKCHNP
IRWYQNIPVLSWLVLRGKCSNCQNLISVRYPMIEVLTAVCALIVLAVFGPTVQMLFGLLLTYTLIALTFIDFDTQLLPDR
YTLPLAALGLAVNSYAIYTSPTSAIWGYVIGFLCLWVVYYLFKIVTGKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSL
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=547652 J4G45_RS13760 WP_004975862.1 2432231..2433091(+) (pilD) [Acinetobacter towneri strain GX5]
ATGCAAGAATTAATCGCGTACTTTATCCAAAATCCAACTGCGCTCTATATCGCAGTTGGACTTTTTAGTTTATGTATTGG
CAGTTTTTTGAATGTGGTGATTTACCGCACCCCAAAAATGATGGAACAGGAGTGGCGTAATGACTGCCAAATGTTATTAC
ATCCTGAACAAGCCATTATTGATGAAAGTAAAATCACCCTGAGCAAACCTGCATCGACCTGCCCCAAATGTCACAACCCA
ATTCGTTGGTATCAAAATATTCCTGTGCTGAGTTGGTTGGTATTACGCGGTAAATGTAGCAACTGCCAAAACCTGATCAG
CGTACGTTATCCAATGATTGAAGTATTAACCGCTGTTTGCGCCCTGATTGTGCTTGCGGTATTTGGCCCAACTGTACAAA
TGCTGTTTGGTTTGCTGCTCACTTATACCTTGATTGCACTCACCTTCATTGATTTTGATACACAACTCTTGCCAGACCGT
TACACCCTGCCTTTGGCTGCACTGGGTTTGGCAGTCAACAGCTATGCCATCTATACCTCACCGACATCGGCAATTTGGGG
TTATGTAATTGGCTTCCTATGTCTATGGGTGGTGTATTACCTGTTTAAAATCGTCACAGGCAAAGAAGGCATGGGCTATG
GTGACTTCAAGCTCCTTGCCGCTTTAGGTGCATGGATGGGGCCGATGATGTTACCCCTGATTGTGCTGCTATCGTCCTTA
GTTGGGGCTATTATTGGCATTATTCTGTTAAAAGTGCGTAAAGAAAACCAACCCTTTGCATTTGGTCCGTATATCGCCAT
TGCGGGCTGGATTGCCTTTTTATGGGGCGAGCAAATCATGAAAGTATATTTGGGACAATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R0DGY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

80.351

99.65

0.801
  pilD Acinetobacter baumannii D1279779

80

99.65

0.797
  pilD Vibrio cholerae strain A1552

55.224

93.706

0.517
  pilD Vibrio campbellii strain DS40M4

52.788

94.056

0.497
  pilD Neisseria gonorrhoeae MS11

46.154

95.455

0.441