Detailed information NTG0054753

Bioinformatically predicted

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: PLE7327_RS03035
• Genome accession: NC_019689
• Coordinates: 692856..695462 (-)
• Length: 868 a.a.
• NCBI ID: WP_015142391.1
• Uniprot ID: K9T0W3
• Organism: Pleurocapsa sp. PCC 7327
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 687856..700462

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PLE7327_RS03015 (Ple7327_0646)	-	688633..689106 (-)	474	WP_015142387.1	hypothetical protein	-
PLE7327_RS03020 (Ple7327_0647)	-	689505..690956 (+)	1452	WP_015142388.1	BCD family MFS transporter	-
PLE7327_RS03025 (Ple7327_0648)	miaA	691025..691939 (+)	915	WP_015142389.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
PLE7327_RS03030 (Ple7327_0649)	-	691962..692756 (-)	795	WP_015142390.1	MlaE family lipid ABC transporter permease subunit	-
PLE7327_RS03035 (Ple7327_0650)	pilJ	692856..695462 (-)	2607	WP_015142391.1	HAMP domain-containing methyl-accepting chemotaxis protein	Machinery gene
PLE7327_RS03040 (Ple7327_0651)	-	695641..696177 (-)	537	WP_015142392.1	chemotaxis protein CheW	-
PLE7327_RS03045 (Ple7327_0652)	pilH	696186..696551 (-)	366	WP_015142393.1	PleD family two-component system response regulator	Machinery gene
PLE7327_RS03050 (Ple7327_0653)	-	696780..698006 (-)	1227	WP_015142394.1	response regulator	-
PLE7327_RS03055 (Ple7327_0654)	hmpF	698506..700119 (+)	1614	WP_015142395.1	pilus motility taxis protein HmpF	-

Sequence

Protein

• Length: 868 a.a.
• Molecular weight: 93984.05 Da
• Isoelectric Point: 3.9816

>NTDB_id=54753 PLE7327_RS03035 WP_015142391.1 692856..695462(-) (pilJ) [Pleurocapsa sp. PCC 7327]
MAPETDYTQLYEQAQTAYLEADYPQAAAIVERLATEYPDDPNVLLLRGHIYLGLHQYALASEQYRTVLELTEEPEWVEYA
QKGLEQAQQMHSAEADNFVDEEATGFTLNESELSATDLSSEENNSFPRWQEESDGITWENNISGGEIDWDSSVFDEDDAG
EPTVGQVQSFDNSFENDLDANFESPSEPSEASSDTPFAQSDDPDTALNWQQLEENPPEFSFAREDRTDYSFSPSQAVGEP
TFVVGTDSQNNPLQDSNEQAKDEFKEEGAIDNPSENFPLEESQDRSAYAVTSTESSESFLDNLDALPDDGLEDITRFDLT
DVAADFSDSELFTSASPELSGNLNSGISQSMASGSGTGLATWGEGESSAALGLSGVSEQFIKPTVEVRQGKLARFLNASF
RQKQWIVAGAAGLTSALAVLLTLGIVSSTASGEAKKNLSPIGGTGLLMTLVTGAASLGATAFLGQVAASQAKRSSDDLQA
QFDAMTAGNFNVKATVYSEDELGQLAAKFTQMARVIATTTSEAQRRATEMEQAKEDLQRQVIRLLDDVEGAARGDLTVEA
EVTADVLGAVADAFNLTIQNLREIVLQVKNAAKQVNKGSTDSELFARNQSSDALRMAEELAVTLNSVQMMTESIQRVAEN
AREAEEVARSSSVTALKGGEAVERTVAGILQIRETVSETARKVKRLAEASQEISKIVALISQIASRTNLLALNASIQAAR
AGEAGRGFAIVADEVRQLADRSAKSLKEIEQIVLQIQSETGSVMTAMEEGIQQVIDVTDKSEQAKRSLEDIIEVSNRIDT
LVRSITADTVKQRENSRSVAQVMQAVELTAQETSQESQRVAGSLQTLVSISRELLTSVERFRVEKGES

Nucleotide

• Length: 2607 bp

>NTDB_id=54753 PLE7327_RS03035 WP_015142391.1 692856..695462(-) (pilJ) [Pleurocapsa sp. PCC 7327]
ATGGCACCAGAGACTGATTATACACAATTGTACGAACAAGCTCAAACAGCTTATCTAGAAGCTGATTATCCACAAGCAGC
CGCAATTGTAGAGCGCTTGGCAACAGAGTATCCAGACGATCCCAACGTATTGTTGTTGCGAGGTCATATCTATCTAGGCT
TGCACCAATACGCTTTAGCAAGCGAGCAATATCGAACAGTTTTAGAACTGACCGAGGAGCCAGAATGGGTTGAATACGCT
CAAAAAGGTCTAGAACAAGCTCAACAGATGCACTCAGCCGAGGCAGACAATTTTGTCGACGAAGAAGCGACAGGATTTAC
CCTCAACGAATCAGAGCTGAGCGCAACCGACCTATCTTCAGAGGAGAATAACAGCTTTCCCCGCTGGCAAGAGGAGTCTG
ATGGCATTACCTGGGAAAACAATATCTCAGGTGGCGAGATAGATTGGGACAGTTCTGTCTTTGATGAAGACGACGCGGGA
GAGCCGACAGTCGGACAAGTGCAATCCTTCGACAATTCTTTTGAAAACGACCTCGATGCCAATTTTGAATCTCCCAGCGA
GCCATCCGAGGCTTCTTCCGATACCCCTTTTGCGCAATCAGACGATCCGGATACCGCCCTCAACTGGCAACAATTAGAGG
AAAACCCCCCCGAATTTTCCTTTGCTCGAGAAGATCGAACGGATTACAGTTTTTCTCCATCTCAAGCCGTCGGAGAACCG
ACTTTTGTCGTCGGCACCGATTCTCAAAACAACCCTTTGCAGGACTCGAACGAGCAAGCAAAAGACGAATTTAAGGAGGA
GGGGGCGATCGACAACCCCAGCGAAAATTTCCCGCTCGAAGAGTCTCAAGATCGATCTGCCTACGCAGTTACCAGCACTG
AATCGAGCGAAAGTTTCCTAGATAATTTAGATGCCCTGCCCGATGACGGATTAGAGGACATCACCAGATTCGACCTGACA
GACGTTGCAGCAGACTTTTCCGATTCCGAGTTGTTTACCTCTGCTAGCCCGGAGTTGAGCGGCAATCTCAATTCGGGAAT
CTCCCAATCGATGGCTTCCGGTAGCGGAACGGGTCTGGCAACTTGGGGCGAGGGAGAGAGCAGTGCCGCTCTCGGTTTGA
GCGGGGTTTCCGAACAATTTATCAAACCGACTGTTGAGGTCAGACAAGGGAAACTCGCAAGATTTCTCAATGCTTCCTTC
AGGCAAAAACAGTGGATTGTCGCTGGAGCAGCAGGACTGACTTCAGCCTTAGCCGTCCTCCTGACGCTTGGCATCGTCTC
CTCTACAGCGTCTGGAGAGGCTAAAAAAAACCTTTCCCCTATTGGCGGGACGGGGTTGTTAATGACATTGGTGACAGGGG
CGGCTAGCTTAGGCGCGACGGCATTCTTAGGACAGGTGGCTGCCAGTCAAGCCAAGCGCTCTAGCGACGACCTGCAAGCC
CAGTTCGATGCAATGACAGCAGGCAATTTTAACGTTAAGGCAACGGTATACTCAGAAGACGAACTGGGGCAACTAGCGGC
AAAATTCACTCAGATGGCGCGGGTCATCGCTACAACGACCAGCGAAGCGCAACGGCGAGCGACTGAGATGGAACAAGCAA
AGGAAGACTTGCAGCGCCAAGTAATTCGCTTACTCGACGATGTAGAAGGAGCGGCGCGAGGCGATCTGACGGTAGAAGCG
GAAGTGACTGCCGACGTGTTGGGGGCGGTTGCCGATGCTTTCAACCTGACCATTCAAAATCTGCGAGAAATCGTCCTCCA
GGTAAAAAATGCCGCCAAGCAAGTAAACAAGGGATCTACCGATAGCGAACTGTTTGCCCGCAACCAGTCTAGCGACGCGC
TGCGAATGGCGGAGGAATTGGCAGTGACGCTCAATTCGGTACAGATGATGACCGAATCGATTCAGCGCGTTGCCGAAAAT
GCTCGCGAAGCGGAAGAAGTGGCTCGGTCGTCATCGGTCACTGCTCTCAAGGGCGGCGAAGCGGTAGAGCGAACGGTCGC
AGGCATTCTGCAAATCCGCGAAACGGTATCGGAGACAGCGCGCAAGGTGAAGCGCCTCGCCGAAGCGTCTCAGGAAATTT
CTAAGATCGTCGCTTTGATTTCTCAGATTGCTTCTCGTACCAACCTATTAGCGCTCAACGCTTCGATTCAGGCGGCAAGA
GCGGGAGAAGCGGGCCGGGGTTTCGCGATCGTTGCCGACGAAGTGCGCCAGCTAGCCGATCGCTCGGCAAAATCGTTGAA
GGAAATCGAGCAAATCGTTCTACAAATCCAGAGCGAAACTGGCTCGGTTATGACCGCTATGGAAGAAGGCATCCAGCAGG
TGATCGACGTAACCGATAAGTCAGAGCAGGCAAAGCGATCGCTCGAAGATATCATTGAGGTATCCAACCGTATCGATACG
CTAGTGCGATCGATTACTGCCGATACGGTCAAGCAGCGAGAAAACTCTCGCTCGGTAGCTCAGGTTATGCAGGCGGTAGA
ATTGACCGCCCAGGAAACCTCTCAAGAGTCTCAGCGAGTGGCTGGTTCCTTGCAAACCCTCGTTAGCATCTCTAGAGAAC
TCCTAACCTCGGTGGAACGTTTCCGCGTCGAGAAAGGCGAGAGCTAG

Domains

Predicted by InterproScan.

• HAMP domain (HAMP) (476-514)

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (646-829)

3D structure

• AlphaFold DB: K9T0W3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilJ	Synechocystis sp. PCC 6803	51.94	100	0.555