Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RIV7116_RS00630 Genome accession   NC_019678
Coordinates   154555..156567 (-) Length   670 a.a.
NCBI ID   WP_015116315.1    Uniprot ID   K9R6M6
Organism   Rivularia sp. PCC 7116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 149555..161567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIV7116_RS34615 (Riv7116_0124) - 149881..150941 (+) 1061 Protein_121 IS630 family transposase -
  RIV7116_RS00615 (Riv7116_0125) - 151344..151898 (-) 555 WP_015116312.1 hypothetical protein -
  RIV7116_RS00620 (Riv7116_0126) - 152033..153262 (-) 1230 WP_015116313.1 type II secretion system F family protein -
  RIV7116_RS00625 (Riv7116_0127) pilT 153369..154490 (-) 1122 WP_015116314.1 type IV pilus twitching motility protein PilT Machinery gene
  RIV7116_RS00630 (Riv7116_0128) pilB/pilB1 154555..156567 (-) 2013 WP_015116315.1 GspE/PulE family protein Machinery gene
  RIV7116_RS00635 (Riv7116_0129) grpE 157101..157760 (+) 660 WP_015116316.1 nucleotide exchange factor GrpE -
  RIV7116_RS00640 (Riv7116_0130) dnaK 158028..160016 (+) 1989 WP_015116317.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74916.41 Da        Isoelectric Point: 4.8225

>NTDB_id=54669 RIV7116_RS00630 WP_015116315.1 154555..156567(-) (pilB/pilB1) [Rivularia sp. PCC 7116]
MTYSSQQRRSTALSVKSEFSPFGNKLVQSGFVDNSQMKQALIESRKSGRPLIEVIESIAGRQLSPELLREYKKQQLFELK
ILYGVESLDPEVSQVGTTSVGGLIETLVPVDICRRHRLIPLSKHEDENPPYILVAMVDPDNLEASDDLNRILRPQGFSLQ
RMVITAEDYQQLINQFLDEMAVKQKHIEKQQFTDINQDLENLDNLDLEDAPEEAEADLGSAMKGAEDAPIINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIVPAITARFKIIATLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDTETLQIVQEMVSRPFGLILVTGPTGSGKTTSLYSALAERNSPDINISTVE
DPVEYSLPGITQVQVIREKGLDFGTALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIESFMISSSLIGVLAQRLMRRVCSECRIEYSPTSQELSRYGLSASQEADVTFYKANTLSIEQAQEAKANNNLCEKCNG
IGYKGRCGVYEVMRITERIQALINEEAPTELIKEAAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KSGLTCRNCEAQLQPEWLDCPYCMTSRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=54669 RIV7116_RS00630 WP_015116315.1 154555..156567(-) (pilB/pilB1) [Rivularia sp. PCC 7116]
ATGACTTACTCGTCGCAACAAAGGCGTAGTACCGCTCTGAGCGTTAAATCGGAGTTTTCGCCTTTCGGTAACAAACTTGT
TCAATCTGGCTTCGTTGACAACAGTCAGATGAAGCAGGCACTAATAGAAAGTCGCAAATCGGGTAGACCTTTAATTGAGG
TTATCGAATCGATTGCAGGGCGACAACTATCACCAGAGCTACTTCGCGAATATAAAAAGCAACAGCTATTTGAACTTAAA
ATTCTTTACGGCGTAGAATCCTTAGATCCGGAAGTTAGCCAAGTCGGCACAACTTCTGTGGGGGGCTTGATTGAGACTCT
TGTGCCCGTTGATATTTGTCGTCGGCATCGCCTAATTCCATTGTCAAAACATGAAGATGAGAATCCACCCTACATTTTAG
TAGCGATGGTGGATCCAGATAATCTGGAGGCTTCCGACGACTTGAACCGGATTTTACGTCCTCAAGGCTTCAGTTTACAA
AGAATGGTAATTACCGCTGAGGATTATCAGCAGCTAATCAACCAATTTTTGGATGAAATGGCTGTTAAACAGAAGCATAT
AGAAAAGCAACAATTTACCGATATTAATCAAGATTTAGAAAATCTAGATAATCTCGATTTAGAAGATGCTCCTGAAGAAG
CTGAAGCTGATTTAGGGTCGGCGATGAAAGGTGCTGAGGATGCACCAATTATTAACTTGGTGAATAGAATTCTTGCCAAA
GCATTGCATGAAGGAGTTTCTGATATTCACATCGAACCCCAAGAAGAAAATTTACGTATACGTTTTCGTAAGGATGGTGT
ATTAAGGGAAGCTTTTGACCCCTTACCGAAAAAAATCGTTCCAGCAATTACAGCCCGTTTTAAAATTATTGCGACCTTAG
ATATCGCCGAACGTCGCCTTCCCCAGGATGGACGCATCCGCCGTTTGTTTGAAGGGCGCAAGGTCGATTTCCGCGTAAAT
ACGTTACCAAGTCGTTACGGAGAAAAGGTAGTACTCCGAATTCTTGATAACTCCTCAACTCAATTGGGACTGGATAAATT
AATTACCGATACCGAGACGTTGCAAATTGTTCAGGAAATGGTAAGCCGCCCCTTTGGCTTAATTCTAGTAACAGGGCCTA
CTGGTTCGGGTAAAACAACTTCTCTGTATTCTGCCTTAGCCGAACGAAATTCTCCTGATATTAATATCAGTACCGTGGAA
GACCCGGTGGAATATAGTTTGCCCGGAATTACTCAAGTGCAGGTTATTCGGGAAAAAGGTTTAGATTTCGGTACGGCATT
GCGGGCATTCTTACGACAAGATCCAGATGTATTACTTGTTGGTGAAACCCGAGATAAAGAAACTGCAAAAACAGCTATCG
AAGCAGCACTAACAGGTCACTTAGTATTAACAACCCTGCACACCAACGATGCTCCCGGTGCGATCGCACGTCTTGGAGAA
ATGGGCATTGAATCTTTTATGATTTCTAGCTCCTTAATCGGGGTGCTGGCACAACGTTTAATGCGTCGCGTATGTAGCGA
ATGTCGTATTGAATACTCGCCTACATCGCAAGAACTTAGCCGCTACGGTTTATCGGCATCTCAAGAAGCTGATGTCACTT
TCTATAAAGCTAATACACTCAGCATCGAGCAGGCTCAAGAAGCTAAAGCTAATAACAATCTTTGTGAAAAATGTAACGGC
ATTGGTTACAAAGGACGTTGCGGTGTTTACGAAGTAATGAGAATTACCGAACGCATCCAAGCTTTAATCAACGAGGAAGC
ACCAACAGAATTAATTAAAGAAGCAGCAGTAGAAGAGGGAATGAAAACCTTGCTTGCTTACAGCTTGGATTTGGTGCGTC
AAGGAGCTACTACCCTCGAAGAAGTGGAACGGGTAACATTTACCGACACGGGCTTGGAAGCGGAATTGAAAGCGAAACGT
AAGAGTGGTTTGACTTGCCGAAATTGTGAAGCTCAATTACAACCTGAATGGTTGGATTGTCCTTACTGTATGACATCTCG
CTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9R6M6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.327

100

0.655

Multiple sequence alignment