Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CYAGR_RS11010 Genome accession   NC_019675
Coordinates   2265822..2267279 (+) Length   485 a.a.
NCBI ID   WP_015109888.1    Uniprot ID   K9P918
Organism   Cyanobium gracile PCC 6307     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2260822..2272279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYAGR_RS10980 (Cyagr_2334) murB 2261289..2262182 (+) 894 WP_015109882.1 UDP-N-acetylmuramate dehydrogenase -
  CYAGR_RS10985 (Cyagr_2335) - 2262210..2262551 (+) 342 WP_015109883.1 YbaB/EbfC family nucleoid-associated protein -
  CYAGR_RS10990 (Cyagr_2336) rsgA 2262508..2263596 (-) 1089 WP_015109884.1 ribosome small subunit-dependent GTPase A -
  CYAGR_RS10995 (Cyagr_2337) - 2263586..2263831 (-) 246 WP_015109885.1 sulfurtransferase TusA family protein -
  CYAGR_RS11000 (Cyagr_2338) dnaJ 2263828..2264952 (-) 1125 WP_015109886.1 molecular chaperone DnaJ -
  CYAGR_RS11005 (Cyagr_2339) grpE 2264952..2265686 (-) 735 WP_015109887.1 nucleotide exchange factor GrpE -
  CYAGR_RS11010 (Cyagr_2340) pilB/pilB1 2265822..2267279 (+) 1458 WP_015109888.1 GspE/PulE family protein Machinery gene
  CYAGR_RS11015 (Cyagr_2341) - 2267285..2268364 (+) 1080 Protein_2276 type IV pilus twitching motility protein PilT -
  CYAGR_RS11020 (Cyagr_2342) - 2268373..2269623 (+) 1251 WP_015109889.1 type II secretion system F family protein -
  CYAGR_RS11025 (Cyagr_2343) - 2269627..2270937 (-) 1311 WP_015109890.1 aminoacetone oxidase family FAD-binding enzyme -
  CYAGR_RS11030 (Cyagr_2344) dusA 2270944..2271966 (+) 1023 WP_015109891.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 485 a.a.        Molecular weight: 53280.48 Da        Isoelectric Point: 5.0250

>NTDB_id=54649 CYAGR_RS11010 WP_015109888.1 2265822..2267279(+) (pilB/pilB1) [Cyanobium gracile PCC 6307]
MPSEGLTQRPQVTGAEHTSLFRDLDLGQAATPQGPSTPETEDLEATSSGEGISPVMSLVDRILIESLTSGASDIHVEPQE
DGLLVRFRQDGVLRRIDKLPRTLIPAVTSRFKIMADLDIAERRMPQDGRIRRNFRGRTTDFRVSTLPGRYGEKVVLRLLD
SGATQLGLDTLITDEEARSALRDIGSKPFGMILVTGPTGSGKSTTLYALLSERNDPSINISTVEDPIEYTLQGITQTQVN
REKGYDFSSALRAFMRQDPDVLLVGETRDLETARTAIEAALTGHLVLTTLHCNDAASAFARLDEMGMEPFLVSASLLGIV
SQRLLRRLCSECRIPYHPNHSELARFGLVASDETDISFYRANTVQPGSEGCCSACQGRGYKGRVGVYEILRMNETLSAAV
AKRATTEELRRLALESGMKTLLGYGLDLVREGLTTLEQVERMLLTDTGLESERRARALSTLTCRSCGAGLEDEWLECPYC
LCVRS

Nucleotide


Download         Length: 1458 bp        

>NTDB_id=54649 CYAGR_RS11010 WP_015109888.1 2265822..2267279(+) (pilB/pilB1) [Cyanobium gracile PCC 6307]
ATGCCATCAGAAGGTCTCACCCAGCGCCCGCAGGTCACCGGTGCAGAGCACACCTCGCTCTTCCGCGACCTTGACCTGGG
TCAGGCCGCCACTCCCCAGGGGCCAAGCACACCGGAAACCGAGGACCTGGAAGCCACCAGCTCGGGCGAGGGCATCTCGC
CGGTGATGAGCCTGGTGGATCGGATCCTGATCGAGTCGCTCACCAGCGGCGCCAGCGACATCCACGTGGAACCCCAGGAA
GACGGCCTGCTGGTCCGCTTCCGCCAGGACGGCGTGCTGCGCCGCATCGACAAGCTGCCCCGCACGTTGATTCCCGCGGT
CACCTCCCGCTTCAAGATCATGGCCGACCTGGACATCGCCGAACGGCGGATGCCCCAGGACGGCCGGATCCGCCGCAACT
TCCGCGGCCGCACCACGGACTTCCGGGTCAGCACCCTGCCCGGCCGCTACGGCGAGAAGGTGGTGCTGCGGCTGCTCGAC
AGCGGCGCCACCCAGTTGGGCCTGGACACCCTGATCACCGACGAGGAAGCCCGCTCCGCCTTGCGGGACATCGGCTCCAA
GCCCTTCGGCATGATCCTGGTCACCGGGCCGACGGGTTCCGGCAAGTCGACCACCCTCTACGCCCTGCTCTCGGAGCGCA
ACGACCCCTCGATCAACATCTCCACGGTCGAGGATCCGATCGAATACACCCTCCAGGGCATCACCCAGACCCAGGTGAAC
CGGGAGAAGGGCTACGACTTCAGCTCCGCCCTGCGCGCCTTCATGCGGCAGGACCCGGACGTGCTGCTGGTGGGGGAGAC
CCGCGACCTGGAGACCGCCCGCACCGCCATCGAGGCGGCCCTGACGGGCCACCTGGTGCTCACGACCCTGCACTGCAACG
ACGCCGCCAGCGCCTTTGCCCGGCTCGACGAGATGGGGATGGAGCCCTTCCTGGTGAGCGCCTCACTGCTGGGGATCGTC
TCCCAGCGCCTCCTGCGACGCCTGTGCAGCGAGTGCCGGATCCCCTACCACCCGAACCACAGCGAACTGGCCCGTTTCGG
CCTGGTGGCCTCCGACGAGACCGACATCTCCTTCTACCGCGCCAACACGGTGCAGCCGGGCTCGGAGGGCTGCTGCAGCG
CCTGCCAGGGGCGGGGGTACAAGGGCCGGGTGGGGGTCTACGAGATCCTGCGCATGAACGAGACCCTCTCCGCCGCCGTG
GCCAAGCGGGCGACGACCGAGGAGCTGCGGCGCCTGGCCCTGGAGAGCGGCATGAAAACGCTCCTCGGCTACGGCCTCGA
TCTGGTGCGGGAGGGCCTGACCACCCTGGAGCAGGTGGAGCGCATGCTGCTCACCGACACGGGCCTGGAATCGGAGCGCC
GCGCCCGGGCCCTGAGCACCCTCACCTGCCGCAGCTGCGGGGCCGGCCTGGAGGACGAATGGTTGGAATGTCCCTACTGT
CTGTGCGTTCGGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-327)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9P918

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

62.108

91.959

0.571
  pilB Vibrio campbellii strain DS40M4

46.059

83.711

0.386
  pilB Vibrio parahaemolyticus RIMD 2210633

45.32

83.711

0.379
  pilB Vibrio cholerae strain A1552

46.173

80.825

0.373
  pilB Acinetobacter baumannii D1279779

41.163

88.66

0.365
  pilB Legionella pneumophila strain ERS1305867

43.35

83.711

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.361

80

0.363

Multiple sequence alignment