Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   LOF66_RS12410 Genome accession   NZ_CP087564
Coordinates   2443259..2443918 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain CM2010-0578/1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2438259..2448918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOF66_RS12385 (LOF66_12305) ygiS 2439542..2441149 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  LOF66_RS12390 (LOF66_12310) mqsA 2441282..2441677 (-) 396 WP_274480475.1 type II toxin-antitoxin system antitoxin MqsA -
  LOF66_RS12395 (LOF66_12315) mqsR 2441679..2441975 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  LOF66_RS12400 (LOF66_12320) ygiV 2442180..2442662 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  LOF66_RS12405 (LOF66_12325) ygiW 2442715..2443107 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  LOF66_RS12410 (LOF66_12330) ciaR 2443259..2443918 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  LOF66_RS12415 (LOF66_12335) qseC 2443915..2445264 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  LOF66_RS12420 (LOF66_12340) ygiZ 2445310..2445642 (-) 333 WP_000917684.1 DUF2645 family protein -
  LOF66_RS12425 (LOF66_12345) mdaB 2445961..2446542 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  LOF66_RS12430 (LOF66_12350) ygiN 2446573..2446887 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  LOF66_RS12435 (LOF66_12355) parE 2446935..2448827 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=546090 LOF66_RS12410 WP_001221493.1 2443259..2443918(+) (ciaR) [Escherichia coli strain CM2010-0578/1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=546090 LOF66_RS12410 WP_001221493.1 2443259..2443918(+) (ciaR) [Escherichia coli strain CM2010-0578/1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365