Detailed information NTG0544794

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: J0J35_RS02410
• Genome accession: NZ_CP071301
• Coordinates: 459653..460909 (-)
• Length: 418 a.a.
• NCBI ID: WP_206917518.1
• Uniprot ID: -
• Organism: Lactococcus sp. LG606
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 454653..465909

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J0J35_RS02380 (J0J35_02385)	-	455406..456512 (+)	1107	WP_206917513.1	alanine dehydrogenase	-
J0J35_RS02385 (J0J35_02390)	-	456632..457591 (+)	960	WP_206917515.1	PhoH family protein	-
J0J35_RS02390 (J0J35_02395)	-	457593..458066 (+)	474	WP_117517938.1	NUDIX hydrolase	-
J0J35_RS02395 (J0J35_02400)	ybeY	458076..458564 (+)	489	WP_019293561.1	rRNA maturation RNase YbeY	-
J0J35_RS02400 (J0J35_02405)	-	458548..459009 (+)	462	WP_019292772.1	diacylglycerol kinase family protein	-
J0J35_RS02405 (J0J35_02410)	-	459012..459656 (-)	645	WP_206917517.1	ComF family protein	-
J0J35_RS02410 (J0J35_02415)	comFA	459653..460909 (-)	1257	WP_206917518.1	DEAD/DEAH box helicase	Machinery gene
J0J35_RS02415 (J0J35_02420)	-	460964..461587 (+)	624	WP_019292769.1	YigZ family protein	-
J0J35_RS02420 (J0J35_02425)	-	461600..462622 (+)	1023	WP_019293557.1	DUF475 domain-containing protein	-
J0J35_RS02425 (J0J35_02430)	-	462657..463679 (-)	1023	WP_206917520.1	alpha/beta hydrolase	-
J0J35_RS02430 (J0J35_02435)	-	463997..464776 (+)	780	WP_019292766.1	formate/nitrite transporter family protein	-
J0J35_RS02435 (J0J35_02440)	glyQ	464790..465758 (+)	969	WP_019292765.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47941.76 Da
• Isoelectric Point: 9.9908

>NTDB_id=544794 J0J35_RS02410 WP_206917518.1 459653..460909(-) (comFA) [Lactococcus sp. LG606]
MNELYGRLLLQKELSKIPDKATLFDSMHDISKTVIMCNRCGKKSKKKEVLLPVGAYYCPYCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLILPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLSQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGEVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=544794 J0J35_RS02410 WP_206917518.1 459653..460909(-) (comFA) [Lactococcus sp. LG606]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAGTTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAAAACAGTGATTATGTGCAATCGCTGCGGTAAAAAAAGTAAGAAAAAAGAGGTCTTACTCCCCGTTG
GTGCTTATTATTGTCCCTATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCACCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTGCAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGACA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGTCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGATCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTCTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAATAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGATACTCCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAGCCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGAAGAGTAGGTCGAAGTACCGAAAGACCTGATGGCGAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (97-211)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.143	93.78	0.536
comFA/cflA	Streptococcus pneumoniae TIGR4	51.522	100	0.526
comFA/cflA	Streptococcus pneumoniae Rx1	51.522	100	0.526
comFA/cflA	Streptococcus pneumoniae D39	51.522	100	0.526
comFA/cflA	Streptococcus pneumoniae R6	51.522	100	0.526
comFA/cflA	Streptococcus mitis SK321	51.054	100	0.522
comFA/cflA	Streptococcus mitis NCTC 12261	50.117	100	0.512
comFA	Bacillus subtilis subsp. subtilis str. 168	37.671	100	0.395
comFA	Latilactobacillus sakei subsp. sakei 23K	38.791	94.976	0.368