Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   J0J33_RS06485 Genome accession   NZ_CP071295
Coordinates   1306120..1307376 (-) Length   418 a.a.
NCBI ID   WP_206888838.1    Uniprot ID   -
Organism   Lactococcus sp. LG1267     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1301120..1312376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J33_RS06455 (J0J33_06455) ald 1301873..1302979 (+) 1107 WP_206888834.1 alanine dehydrogenase -
  J0J33_RS06460 (J0J33_06460) - 1303099..1304058 (+) 960 WP_004257953.1 PhoH family protein -
  J0J33_RS10945 (J0J33_06465) - 1304060..1304533 (+) 474 Protein_1223 NUDIX hydrolase -
  J0J33_RS06470 (J0J33_06470) ybeY 1304543..1305031 (+) 489 WP_019292773.1 rRNA maturation RNase YbeY -
  J0J33_RS06475 (J0J33_06475) - 1305015..1305476 (+) 462 WP_019292772.1 diacylglycerol kinase family protein -
  J0J33_RS06480 (J0J33_06480) - 1305479..1306123 (-) 645 WP_206888836.1 ComF family protein -
  J0J33_RS06485 (J0J33_06485) comFA 1306120..1307376 (-) 1257 WP_206888838.1 DEAD/DEAH box helicase Machinery gene
  J0J33_RS06490 (J0J33_06490) - 1307431..1308054 (+) 624 WP_019292769.1 YigZ family protein -
  J0J33_RS06495 (J0J33_06495) - 1308067..1309089 (+) 1023 WP_206921626.1 DUF475 domain-containing protein -
  J0J33_RS06500 (J0J33_06500) - 1309124..1310146 (-) 1023 WP_034999659.1 alpha/beta hydrolase -
  J0J33_RS06505 (J0J33_06505) - 1310464..1311243 (+) 780 WP_019292766.1 formate/nitrite transporter family protein -
  J0J33_RS06510 (J0J33_06510) glyQ 1311257..1312225 (+) 969 WP_165719545.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47941.77 Da        Isoelectric Point: 10.1309

>NTDB_id=544732 J0J33_RS06485 WP_206888838.1 1306120..1307376(-) (comFA) [Lactococcus sp. LG1267]
MNELYGRLLLQKELSKIPDKATLFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLVILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLNQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=544732 J0J33_RS06485 WP_206888838.1 1306120..1307376(-) (comFA) [Lactococcus sp. LG1267]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTGCAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGTCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTAGTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTCTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAATAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAACCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGCCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCTGGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(274-382)

Type III restriction enzyme, res subunit (ResIII)

(97-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.143

93.78

0.536
  comFA/cflA Streptococcus pneumoniae TIGR4

50.82

100

0.519
  comFA/cflA Streptococcus pneumoniae Rx1

50.82

100

0.519
  comFA/cflA Streptococcus pneumoniae D39

50.82

100

0.519
  comFA/cflA Streptococcus pneumoniae R6

50.82

100

0.519
  comFA/cflA Streptococcus mitis SK321

50.351

100

0.514
  comFA/cflA Streptococcus mitis NCTC 12261

49.415

100

0.505
  comFA Bacillus subtilis subsp. subtilis str. 168

37.443

100

0.392
  comFA Latilactobacillus sakei subsp. sakei 23K

39.043

94.976

0.371