Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   J0J36_RS04235 Genome accession   NZ_CP071293
Coordinates   838976..840232 (-) Length   418 a.a.
NCBI ID   WP_153924719.1    Uniprot ID   -
Organism   Lactococcus sp. LG1074     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 833976..845232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J36_RS04205 (J0J36_04205) - 834741..835835 (+) 1095 WP_153924713.1 alanine dehydrogenase -
  J0J36_RS04210 (J0J36_04210) - 835955..836914 (+) 960 WP_153924716.1 PhoH family protein -
  J0J36_RS04215 (J0J36_04215) - 836916..837389 (+) 474 WP_019292774.1 NUDIX hydrolase -
  J0J36_RS04220 (J0J36_04220) ybeY 837399..837887 (+) 489 WP_019292773.1 rRNA maturation RNase YbeY -
  J0J36_RS04225 (J0J36_04225) - 837871..838332 (+) 462 WP_019292772.1 diacylglycerol kinase family protein -
  J0J36_RS04230 (J0J36_04230) - 838335..838979 (-) 645 WP_019292771.1 ComF family protein -
  J0J36_RS04235 (J0J36_04235) comFA 838976..840232 (-) 1257 WP_153924719.1 DEAD/DEAH box helicase Machinery gene
  J0J36_RS04240 (J0J36_04240) - 840287..840910 (+) 624 WP_019292769.1 YigZ family protein -
  J0J36_RS04245 (J0J36_04245) - 840923..841945 (+) 1023 WP_153924722.1 DUF475 domain-containing protein -
  J0J36_RS04250 (J0J36_04250) - 841980..843002 (-) 1023 WP_153924725.1 alpha/beta hydrolase -
  J0J36_RS04255 (J0J36_04255) - 843320..844099 (+) 780 WP_019292766.1 formate/nitrite transporter family protein -
  J0J36_RS04260 (J0J36_04260) glyQ 844113..845081 (+) 969 WP_019292765.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47922.80 Da        Isoelectric Point: 10.1309

>NTDB_id=544677 J0J36_RS04235 WP_153924719.1 838976..840232(-) (comFA) [Lactococcus sp. LG1074]
MNELYGRLLLQKELSKIPDKATLFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVALTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMSFRHAFDLLILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTDN
LERQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLNQDLQSSFPHEKIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKITA
MLEAYKNIRNINKAGGFQ

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=544677 J0J36_RS04235 WP_153924719.1 838976..840232(-) (comFA) [Lactococcus sp. LG1074]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTACAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGCTTTAACTAGCCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGAGTTTTCGTCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTTTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCGATAAT
TTGGAAAGACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAACCAAGACTTACAGAGTAGTTTTCCTCATGAAAAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGCCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCTGGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGTTTTTCTTTCATTTAGGAAAAATAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATAAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(97-236)

Helicase conserved C-terminal domain (Helicase_C)

(274-382)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

56.888

93.78

0.533
  comFA/cflA Streptococcus mitis SK321

51.054

100

0.522
  comFA/cflA Streptococcus pneumoniae TIGR4

50.585

100

0.517
  comFA/cflA Streptococcus pneumoniae Rx1

50.585

100

0.517
  comFA/cflA Streptococcus pneumoniae D39

50.585

100

0.517
  comFA/cflA Streptococcus pneumoniae R6

50.585

100

0.517
  comFA/cflA Streptococcus mitis NCTC 12261

50.117

100

0.512
  comFA Bacillus subtilis subsp. subtilis str. 168

36.986

100

0.388
  comFA Latilactobacillus sakei subsp. sakei 23K

38.791

94.976

0.368