Detailed information NTG0544588

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: J0J32_RS04165
• Genome accession: NZ_CP071285
• Coordinates: 835221..836477 (-)
• Length: 418 a.a.
• NCBI ID: WP_004257939.1
• Uniprot ID: -
• Organism: Lactococcus garvieae strain LG728
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 830221..841477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J0J32_RS04130 (J0J32_04130)	rpmA	830340..830624 (+)	285	WP_004257956.1	50S ribosomal protein L27	-
J0J32_RS04135 (J0J32_04135)	-	830976..832082 (+)	1107	WP_004257955.1	alanine dehydrogenase	-
J0J32_RS04140 (J0J32_04140)	-	832200..833159 (+)	960	WP_207144564.1	PhoH family protein	-
J0J32_RS04145 (J0J32_04145)	-	833161..833634 (+)	474	WP_004257950.1	NUDIX hydrolase	-
J0J32_RS04150 (J0J32_04150)	ybeY	833644..834132 (+)	489	WP_004257946.1	rRNA maturation RNase YbeY	-
J0J32_RS04155 (J0J32_04155)	-	834116..834577 (+)	462	WP_004257944.1	diacylglycerol kinase family protein	-
J0J32_RS04160 (J0J32_04160)	-	834580..835224 (-)	645	WP_004257941.1	ComF family protein	-
J0J32_RS04165 (J0J32_04165)	comFA	835221..836477 (-)	1257	WP_004257939.1	DEAD/DEAH box helicase	Machinery gene
J0J32_RS04170 (J0J32_04170)	-	836532..837155 (+)	624	WP_004257938.1	YigZ family protein	-
J0J32_RS04175 (J0J32_04175)	-	837169..838191 (+)	1023	WP_004257934.1	DUF475 domain-containing protein	-
J0J32_RS04180 (J0J32_04180)	-	838221..839243 (-)	1023	WP_004257931.1	alpha/beta hydrolase	-
J0J32_RS04185 (J0J32_04185)	-	839562..840341 (+)	780	WP_004257929.1	formate/nitrite transporter family protein	-
J0J32_RS04190 (J0J32_04190)	glyQ	840355..841323 (+)	969	WP_004257927.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47861.62 Da
• Isoelectric Point: 9.9268

>NTDB_id=544588 J0J32_RS04165 WP_004257939.1 835221..836477(-) (comFA) [Lactococcus garvieae strain LG728]
MDELYGRLLLQKELTKLPDKAILFDGMQDVSQTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASFLNWQGKLTASQQHVSDSLVNLHQQQKTVLVQAVTGAGKTEMIYQSINNTLKKGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATAHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFALTQARKPSSSLIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide

• Length: 1257 bp

>NTDB_id=544588 J0J32_RS04165 WP_004257939.1 835221..836477(-) (comFA) [Lactococcus garvieae strain LG728]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAATACTTTTTGATGGGAT
GCAAGATGTTTCCCAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATTAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCTCATTGTATTCAAATGGGACGTGTTCGCTCGGACGAAAAACTTTACCACCTTCCACAGGAGGAT
TTTTCAGCAGCTTCTTTTCTCAACTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAATTT
GCATCAGCAACAAAAAACAGTACTGGTACAGGCGGTTACTGGTGCAGGAAAAACTGAAATGATTTATCAGAGCATTAATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCACCACTCGTAATTGCAACGGC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGCGCTGACTCAAGCCCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAATTAGGACAAATCGAGAAGCTACAGCTCCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAGTCTGGTTTTCCCTTACTTATCTTTGTCC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTCGCGTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATTTAGGAAAAACTACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGAGGGTTCTTATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (92-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.143	95.455	0.545
comFA/cflA	Streptococcus mitis SK321	51.878	100	0.529
comFA/cflA	Streptococcus pneumoniae TIGR4	51.878	100	0.529
comFA/cflA	Streptococcus pneumoniae Rx1	51.878	100	0.529
comFA/cflA	Streptococcus pneumoniae D39	51.878	100	0.529
comFA/cflA	Streptococcus pneumoniae R6	51.878	100	0.529
comFA/cflA	Streptococcus mitis NCTC 12261	50.939	100	0.519
comFA	Bacillus subtilis subsp. subtilis str. 168	38.791	94.976	0.368
comFA	Latilactobacillus sakei subsp. sakei 23K	38.287	94.976	0.364