Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   LMF34_RS13445 Genome accession   NZ_CP086678
Coordinates   2808090..2808749 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain EFF60     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2803090..2813749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMF34_RS13420 - 2803832..2804779 (+) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -
  LMF34_RS13425 - 2804776..2805663 (+) 888 WP_000614954.1 MurR/RpiR family transcriptional regulator -
  LMF34_RS13430 ygiN 2805708..2806022 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  LMF34_RS13435 mdaB 2806053..2806634 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  LMF34_RS13440 qseC 2806744..2808093 (-) 1350 WP_248422648.1 quorum sensing histidine kinase QseC -
  LMF34_RS13445 ciaR 2808090..2808749 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  LMF34_RS13450 ygiW 2808901..2809293 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  LMF34_RS13455 ygiV 2809346..2809828 (+) 483 WP_000183492.1 AraC family transcriptional regulator -
  LMF34_RS13460 ygiS 2809937..2811544 (+) 1608 WP_001327858.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=544040 LMF34_RS13445 WP_001221493.1 2808090..2808749(-) (ciaR) [Escherichia coli strain EFF60]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=544040 LMF34_RS13445 WP_001221493.1 2808090..2808749(-) (ciaR) [Escherichia coli strain EFF60]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365