Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   THEOS_RS06165 Genome accession   NC_019386
Coordinates   1183965..1184435 (+) Length   156 a.a.
NCBI ID   WP_016329465.1    Uniprot ID   K7QV49
Organism   Thermus oshimai JL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1178965..1189435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEOS_RS06140 (Theos_1230) ilvN 1179200..1179712 (-) 513 WP_016329460.1 acetolactate synthase small subunit -
  THEOS_RS06145 (Theos_1231) ilvB 1179709..1181397 (-) 1689 WP_016329461.1 biosynthetic-type acetolactate synthase large subunit -
  THEOS_RS06150 (Theos_1232) - 1181543..1182529 (-) 987 WP_016329462.1 YpdA family putative bacillithiol disulfide reductase -
  THEOS_RS06155 (Theos_1233) - 1182554..1183312 (+) 759 WP_016329463.1 TerC family protein -
  THEOS_RS13040 (Theos_1234) - 1183309..1183968 (+) 660 WP_016329464.1 gp58-like family protein -
  THEOS_RS06165 (Theos_1235) pilA/pilA1 1183965..1184435 (+) 471 WP_016329465.1 GspH/FimT family protein Machinery gene
  THEOS_RS06170 (Theos_1236) - 1184490..1184957 (+) 468 WP_016329466.1 GspH/FimT family protein -
  THEOS_RS06175 (Theos_1237) pilA/pilA2 1184954..1185514 (+) 561 WP_016329467.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  THEOS_RS06180 (Theos_1238) pilA/pilA3 1185511..1186203 (+) 693 WP_016329468.1 PilW family protein Machinery gene
  THEOS_RS06185 (Theos_1239) comZ 1186212..1187900 (+) 1689 WP_016329469.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  THEOS_RS14035 (Theos_1240) - 1188131..1188505 (+) 375 WP_016329470.1 type II secretion system protein -
  THEOS_RS14320 - 1188666..1188740 (+) 75 WP_081593791.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEOS_RS06195 (Theos_1241) - 1188785..1189012 (+) 228 WP_051008282.1 hypothetical protein -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16642.24 Da        Isoelectric Point: 9.8795

>NTDB_id=54358 THEOS_RS06165 WP_016329465.1 1183965..1184435(+) (pilA/pilA1) [Thermus oshimai JL-2]
MRGFSLLELLLVLSILGVVLALGLPRLNPDAQAVNQAARGLAEQVVRARLEAIRQNAPAGLAVFTEGAGGYAVFLDQNEN
RAYDPGEEIQAVRFGEGPWARVRLLEEASALGNLPLLFDARGVPAKPIAATIALADPGRRQVRKVVVSQAGRARLE

Nucleotide


Download         Length: 471 bp        

>NTDB_id=54358 THEOS_RS06165 WP_016329465.1 1183965..1184435(+) (pilA/pilA1) [Thermus oshimai JL-2]
GTGAGGGGATTTTCCCTGCTGGAGCTTCTCCTGGTGCTCTCCATCCTGGGGGTCGTCCTGGCCCTGGGCCTCCCCCGCCT
GAACCCCGACGCCCAGGCGGTGAACCAGGCCGCCCGGGGCCTCGCGGAACAGGTGGTCCGCGCGCGCCTCGAGGCCATCC
GCCAGAACGCCCCCGCGGGGCTTGCGGTCTTCACCGAAGGCGCCGGAGGCTACGCGGTCTTCCTGGACCAAAACGAAAAC
CGGGCCTACGACCCCGGGGAAGAGATCCAGGCGGTGCGCTTTGGGGAAGGTCCCTGGGCCCGGGTGCGGCTCCTAGAGGA
AGCGAGCGCCCTGGGCAACCTCCCCCTCCTCTTTGACGCCCGGGGGGTGCCGGCCAAGCCCATCGCCGCCACCATCGCCC
TGGCCGACCCCGGAAGGCGGCAGGTGCGGAAGGTGGTGGTGAGCCAGGCGGGGCGGGCCCGGCTGGAGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-22)

Type II transport protein GspH (GspH)

(37-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K7QV49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Thermus thermophilus HB27

67.442

82.692

0.558

Multiple sequence alignment