Detailed information NTG0054360

Bioinformatically predicted

Overview

• Name: pilA/pilA3
• Type: Machinery gene
• Locus tag: THEOS_RS06180
• Genome accession: NC_019386
• Coordinates: 1185511..1186203 (+)
• Length: 230 a.a.
• NCBI ID: WP_016329468.1
• Uniprot ID: K7R5U3
• Organism: Thermus oshimai JL-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1180511..1191203

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THEOS_RS06150 (Theos_1232)	-	1181543..1182529 (-)	987	WP_016329462.1	YpdA family putative bacillithiol disulfide reductase	-
THEOS_RS06155 (Theos_1233)	-	1182554..1183312 (+)	759	WP_016329463.1	TerC family protein	-
THEOS_RS13040 (Theos_1234)	-	1183309..1183968 (+)	660	WP_016329464.1	gp58-like family protein	-
THEOS_RS06165 (Theos_1235)	pilA/pilA1	1183965..1184435 (+)	471	WP_016329465.1	GspH/FimT family protein	Machinery gene
THEOS_RS06170 (Theos_1236)	-	1184490..1184957 (+)	468	WP_016329466.1	GspH/FimT family protein	-
THEOS_RS06175 (Theos_1237)	pilA/pilA2	1184954..1185514 (+)	561	WP_016329467.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
THEOS_RS06180 (Theos_1238)	pilA/pilA3	1185511..1186203 (+)	693	WP_016329468.1	PilW family protein	Machinery gene
THEOS_RS06185 (Theos_1239)	comZ	1186212..1187900 (+)	1689	WP_016329469.1	PilX N-terminal domain-containing pilus assembly protein	Machinery gene
THEOS_RS14035 (Theos_1240)	-	1188131..1188505 (+)	375	WP_016329470.1	type II secretion system protein	-
THEOS_RS14320	-	1188666..1188740 (+)	75	WP_081593791.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
THEOS_RS06195 (Theos_1241)	-	1188785..1189012 (+)	228	WP_051008282.1	hypothetical protein	-
THEOS_RS06200 (Theos_1242)	-	1189206..1189505 (+)	300	WP_016329472.1	nucleotidyltransferase family protein	-
THEOS_RS06205 (Theos_1243)	-	1189650..1190084 (+)	435	WP_016329473.1	hypothetical protein	-
THEOS_RS06210 (Theos_1244)	-	1190163..1190720 (-)	558	WP_041436195.1	Uma2 family endonuclease	-
THEOS_RS06215 (Theos_1245)	-	1190756..1191058 (+)	303	WP_016329475.1	nucleotidyltransferase family protein	-

Sequence

Protein

• Length: 230 a.a.
• Molecular weight: 24222.70 Da
• Isoelectric Point: 7.9085

>NTDB_id=54360 THEOS_RS06180 WP_016329468.1 1185511..1186203(+) (pilA/pilA3) [Thermus oshimai JL-2]
MRKAGFTLVEVLIALALLVTVLAVALRYFTATAETGRLTQAKGELQDRVRMVMQVVSGDLQLAGAKYWTWGTQSVGFTLV
GTLEGTDGGAKDTLTVRYVTSLREQSAACRRVDYTFSGDTLQRSDVNITPASGAECATGTPSPTPLADGILALDIQYLCS
DGSAKNVPDCGASAYPRSARLTVAGYSLTPVRGGGGSLTTVSGQSLTCPANRLCYAMTQEVLMPNLKPVQ

Nucleotide

• Length: 693 bp

>NTDB_id=54360 THEOS_RS06180 WP_016329468.1 1185511..1186203(+) (pilA/pilA3) [Thermus oshimai JL-2]
ATGAGGAAGGCCGGCTTCACCCTGGTGGAGGTCCTCATCGCCCTGGCCCTCCTGGTGACCGTCCTGGCGGTGGCCCTGCG
CTACTTCACCGCCACCGCGGAAACGGGCCGGCTCACCCAGGCCAAGGGCGAGCTCCAGGACCGGGTGCGCATGGTCATGC
AGGTGGTCTCGGGGGACCTCCAGCTCGCGGGGGCCAAGTACTGGACCTGGGGGACCCAGAGCGTGGGCTTCACCCTGGTG
GGCACCCTCGAGGGCACGGATGGCGGGGCCAAGGACACCCTCACCGTGCGCTACGTGACGAGCCTCCGCGAGCAAAGCGC
CGCCTGCCGCCGGGTGGACTACACCTTTAGCGGGGACACCCTCCAAAGGAGCGACGTGAACATCACCCCCGCAAGCGGCG
CGGAGTGCGCCACCGGCACCCCAAGCCCCACCCCCCTGGCGGACGGCATCCTGGCCCTGGACATCCAGTACCTCTGCTCC
GACGGAAGCGCGAAGAACGTCCCGGACTGCGGCGCCAGCGCCTACCCCCGCTCCGCCCGGCTCACCGTGGCCGGCTACAG
CCTCACCCCGGTGCGGGGGGGTGGGGGGAGCCTCACCACGGTGAGCGGCCAGAGCCTCACCTGCCCTGCGAATAGGCTCT
GCTACGCCATGACCCAGGAAGTCCTCATGCCGAACCTCAAGCCGGTCCAATAG

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (2-26)

3D structure

• AlphaFold DB: K7R5U3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA/pilA3	Thermus thermophilus HB27	65.502	99.565	0.652