Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   JS609_RS12740 Genome accession   NZ_CP071043
Coordinates   2485720..2486337 (-) Length   205 a.a.
NCBI ID   WP_122894878.1    Uniprot ID   -
Organism   Bacillus subtilis isolate ELA2001105     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2480720..2491337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JS609_RS12710 (JS609_02509) rpsT 2481022..2481288 (+) 267 WP_131227637.1 30S ribosomal protein S20 -
  JS609_RS12715 (JS609_02510) holA 2481303..2482346 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  JS609_RS12720 - 2482386..2482535 (-) 150 WP_003229985.1 hypothetical protein -
  JS609_RS12725 yqzM 2482576..2482710 (+) 135 WP_003229983.1 YqzM family protein -
  JS609_RS12730 (JS609_02511) comEC 2482750..2485080 (-) 2331 WP_213414606.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  JS609_RS12735 (JS609_02512) comEB 2485084..2485653 (-) 570 WP_003229978.1 ComE operon protein 2 -
  JS609_RS12740 (JS609_02513) comEA 2485720..2486337 (-) 618 WP_122894878.1 competence protein ComEA Machinery gene
  JS609_RS12745 (JS609_02514) comER 2486421..2487242 (+) 822 WP_213414605.1 late competence protein ComER -
  JS609_RS12750 (JS609_02515) yqeM 2487308..2488051 (-) 744 WP_015384134.1 class I SAM-dependent DNA methyltransferase -
  JS609_RS12755 (JS609_02516) rsfS 2488048..2488404 (-) 357 WP_014477391.1 ribosome silencing factor -
  JS609_RS12760 (JS609_02517) yqeK 2488422..2488982 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  JS609_RS12765 (JS609_02518) nadD 2488972..2489541 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  JS609_RS12770 (JS609_02519) yhbY 2489553..2489843 (-) 291 WP_213414604.1 ribosome assembly RNA-binding protein YhbY -
  JS609_RS12775 (JS609_02520) aroE 2489837..2490679 (-) 843 WP_076458133.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21827.58 Da        Isoelectric Point: 4.9552

>NTDB_id=542527 JS609_RS12740 WP_122894878.1 2485720..2486337(-) (comEA) [Bacillus subtilis isolate ELA2001105]
MNWLNQHKKTIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSKQADEAQVNLAEVLQDGTVVYIPKKGEETAVQQGTGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=542527 JS609_RS12740 WP_122894878.1 2485720..2486337(-) (comEA) [Bacillus subtilis isolate ELA2001105]
ATGAATTGGTTGAATCAGCATAAGAAAACAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCTATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAGTAAACAAGCAGACGAAGCGCAAGTAAATTTGGCCGA
GGTTCTTCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTACCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGGCCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.073

100

0.971
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

36.364

100

0.39