NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	JS608_RS15270	Genome accession	NZ_CP071042
Coordinates	3093380..3093994 (-)	Length	204 a.a.
NCBI ID	WP_015387983.1	Uniprot ID	A0A6A8LHQ3
Organism	Bacillus amyloliquefaciens isolate ELA1901024
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3088380..3098994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JS608_RS15240 (JS608_03050)	rpsT	3088681..3088947 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JS608_RS15245 (JS608_03051)	holA	3088964..3090005 (-)	1042	Protein_2975	DNA polymerase III subunit delta	-
JS608_RS15250	-	3090045..3090199 (-)	155	Protein_2976	hypothetical protein	-
JS608_RS15255	-	3090240..3090374 (+)	135	WP_003152870.1	YqzM family protein	-
JS608_RS15260 (JS608_03052)	-	3090393..3092743 (-)	2351	Protein_2978	DNA internalization-related competence protein ComEC/Rec2	-
JS608_RS15265 (JS608_03053)	-	3092744..3093313 (-)	570	WP_003152868.1	ComE operon protein 2	-
JS608_RS15270 (JS608_03054)	comEA	3093380..3093994 (-)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
JS608_RS15275 (JS608_03055)	comER	3094053..3094874 (+)	822	WP_014305449.1	late competence protein ComER	-
JS608_RS15280 (JS608_03056)	-	3094943..3095680 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
JS608_RS15285 (JS608_03057)	rsfS	3095677..3096033 (-)	357	WP_003152864.1	ribosome silencing factor	-
JS608_RS15290 (JS608_03058)	yqeK	3096030..3096611 (-)	582	WP_063174743.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JS608_RS15295 (JS608_03059)	-	3096601..3097170 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
JS608_RS15300 (JS608_03060)	yhbY	3097181..3097471 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
JS608_RS15305 (JS608_03061)	aroE	3097465..3098307 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22255.41 Da Isoelectric Point: 5.5211

                                >NTDB_id=542457 JS608_RS15270 WP_015387983.1 3093380..3093994(-) (comEA) [Bacillus amyloliquefaciens isolate ELA1901024]

MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=542457 JS608_RS15270 WP_015387983.1 3093380..3093994(-) (comEA) [Bacillus amyloliquefaciens isolate ELA1901024]

ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGTGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCTATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0542457