Detailed information NTG0054065

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: DCF50_RS10100
• Genome accession: NC_018867
• Coordinates: 2098089..2099165 (+)
• Length: 358 a.a.
• NCBI ID: WP_015044083.1
• Uniprot ID: -
• Organism: Dehalobacter sp. CF
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2093089..2104165

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DCF50_RS10085 (DCF50_p2043)	-	2094064..2095440 (+)	1377	WP_242824890.1	ISNCY family transposase	-
DCF50_RS10090 (DCF50_p2044)	-	2095554..2096219 (-)	666	WP_015044081.1	A24 family peptidase	-
DCF50_RS10095 (DCF50_p2045)	-	2096374..2098053 (+)	1680	WP_015044082.1	GspE/PulE family protein	-
DCF50_RS10100 (DCF50_p2046)	pilT	2098089..2099165 (+)	1077	WP_015044083.1	type IV pilus twitching motility protein PilT	Machinery gene
DCF50_RS10105 (DCF50_p2047)	-	2099348..2100559 (-)	1212	WP_015044084.1	hypothetical protein	-
DCF50_RS10110 (DCF50_p2048)	-	2100573..2103326 (-)	2754	WP_015044085.1	methyl-accepting chemotaxis protein	-
DCF50_RS15895 (DCF50_p2049)	-	2103553..2103951 (+)	399	WP_015044086.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 358 a.a.
• Molecular weight: 39782.23 Da
• Isoelectric Point: 6.5013

>NTDB_id=54065 DCF50_RS10100 WP_015044083.1 2098089..2099165(+) (pilT) [Dehalobacter sp. CF]
MNLNIRDLLLMSLELKASDLHITTGLPPMFRINGILTPMANAKYLSPLDTKELVDELMDEPVKNRLQQKGEVDFSYTIPG
ISRFRVNVYSQRKSMAAAIRVIVPDIPSINELGLPDILKDLAMKPRGLILVTGPTGSGKSTTLAAMVRHMNTHRNSHVIT
IEDPIEYLHAHNQCMINQREIGDDTHTFADALRSALREDPDVILVGEMRDLETINTALMASETGHLVLSTLHTSSAATTI
DRIIDVFPPNQLQQIKVQLASVLQGIICQQLLPTLEGTGRVAAMEALIVNDAVRNMIREGKTHQIDTVIQTGIKVGMAPM
DYSLANLVKRKMVTLEEAKSRCVNPELFSKYLDQPYIF

Nucleotide

• Length: 1077 bp

>NTDB_id=54065 DCF50_RS10100 WP_015044083.1 2098089..2099165(+) (pilT) [Dehalobacter sp. CF]
ATGAATCTAAATATCAGGGACTTACTTTTAATGTCATTGGAACTGAAAGCTTCTGACCTCCACATCACAACCGGCCTTCC
CCCAATGTTCAGAATCAATGGTATCCTGACTCCGATGGCAAACGCCAAGTATTTGTCTCCGTTGGACACCAAAGAGCTTG
TCGACGAACTGATGGATGAACCCGTGAAAAACCGTTTGCAGCAAAAGGGAGAAGTTGACTTTTCCTATACGATCCCTGGT
ATCAGCCGTTTCAGGGTTAACGTCTACAGCCAGCGGAAAAGCATGGCAGCCGCGATCAGGGTCATTGTTCCTGATATTCC
AAGCATAAACGAACTGGGACTTCCCGATATCCTGAAAGACCTCGCGATGAAACCCAGAGGTCTGATCCTGGTCACAGGTC
CGACCGGTTCCGGTAAATCCACTACCCTTGCTGCCATGGTGCGCCATATGAATACGCACCGCAACAGTCACGTCATTACC
ATCGAAGACCCGATCGAATATTTGCATGCCCATAATCAATGTATGATAAACCAGCGTGAGATAGGTGACGATACCCATAC
CTTTGCAGATGCTTTACGGTCTGCACTGCGTGAAGACCCCGATGTTATCCTGGTCGGTGAAATGCGCGACCTTGAGACGA
TCAACACGGCCCTGATGGCTTCAGAAACAGGCCACCTGGTCTTGTCCACACTCCATACTTCATCCGCAGCGACTACCATT
GACCGGATCATCGATGTCTTTCCGCCGAATCAGCTGCAGCAAATTAAAGTTCAGCTGGCATCTGTTCTTCAGGGCATTAT
CTGCCAGCAGCTGCTGCCTACTCTTGAGGGTACCGGACGTGTTGCAGCGATGGAAGCTCTCATCGTCAATGACGCTGTCC
GGAATATGATCCGCGAAGGTAAAACCCACCAGATTGATACGGTCATCCAGACTGGCATCAAAGTCGGTATGGCCCCGATG
GATTATTCTCTGGCGAATCTGGTTAAGCGGAAGATGGTAACCCTCGAGGAAGCAAAATCGAGATGTGTGAATCCAGAACT
GTTCTCGAAGTATCTTGATCAGCCGTATATTTTCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (30-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	49.148	98.324	0.483
pilT	Acinetobacter baylyi ADP1	49.133	96.648	0.475
pilT	Neisseria meningitidis 8013	48.991	96.927	0.475
pilT	Acinetobacter baumannii strain A118	48.844	96.648	0.472
pilT	Acinetobacter nosocomialis M2	48.844	96.648	0.472
pilT	Acinetobacter baumannii D1279779	48.844	96.648	0.472
pilT	Neisseria gonorrhoeae MS11	48.703	96.927	0.472
pilT	Vibrio cholerae strain A1552	48.968	94.693	0.464
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.968	94.693	0.464
pilT	Pseudomonas aeruginosa PAK	46.991	97.486	0.458
pilT	Legionella pneumophila strain Lp02	46.705	97.486	0.455
pilT	Legionella pneumophila strain ERS1305867	46.705	97.486	0.455
pilT	Pseudomonas stutzeri DSM 10701	46.132	97.486	0.45
pilU	Pseudomonas stutzeri DSM 10701	41.369	93.855	0.388
pilU	Acinetobacter baylyi ADP1	40.116	96.089	0.385
pilB/pilB1	Synechocystis sp. PCC 6803	29.295	100	0.372
pilU	Vibrio cholerae strain A1552	40.248	90.223	0.363