Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DCF50_RS09435 Genome accession   NC_018867
Coordinates   1960235..1961575 (-) Length   446 a.a.
NCBI ID   WP_015043975.1    Uniprot ID   K4LH30
Organism   Dehalobacter sp. CF     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1955235..1966575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCF50_RS09415 (DCF50_p1926) gltX 1955522..1956979 (-) 1458 WP_015043970.1 glutamate--tRNA ligase -
  DCF50_RS15890 (DCF50_p1927) ispD 1957087..1958274 (-) 1188 WP_015043971.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DCF50_RS09425 (DCF50_p1928) - 1958252..1959418 (-) 1167 WP_015043972.1 PIN/TRAM domain-containing protein -
  DCF50_RS09430 (DCF50_p1930) - 1959689..1960081 (+) 393 WP_015043974.1 hypothetical protein -
  DCF50_RS09435 (DCF50_p1931) radA 1960235..1961575 (-) 1341 WP_015043975.1 DNA repair protein RadA Machinery gene
  DCF50_RS09440 (DCF50_p1932) - 1961589..1964072 (-) 2484 WP_015043976.1 ATP-dependent Clp protease ATP-binding subunit -
  DCF50_RS09445 (DCF50_p1933) - 1964104..1965171 (-) 1068 WP_015043977.1 protein arginine kinase -
  DCF50_RS09450 (DCF50_p1934) - 1965168..1965692 (-) 525 WP_015043978.1 protein-arginine kinase activator protein McsA -
  DCF50_RS09455 (DCF50_p1935) - 1965707..1966180 (-) 474 WP_015043979.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 48963.25 Da        Isoelectric Point: 5.3789

>NTDB_id=54063 DCF50_RS09435 WP_015043975.1 1960235..1961575(-) (radA) [Dehalobacter sp. CF]
MAGPKTKFYCRECGQESARWLGRCPGCGEWNTLIEERVEKSKPTENRGIVQAIPLTEIQTMEGQRLDTGSQELNRVFGGG
VVEGSFVLLSGEPGIGKSTLFLQMAEYLSRKENVLYVSGEESARQIKLRADRMELSSSRVHILTDNSLEAVRSEVLNKGY
KVVFIDSIQTMLLEDVQSAPGSVSQVREGASFLLKLAKENEITVFLAGHITKEGVIAGPRVLEHMVDTVLYFEGDQHHIF
RLLRAVKNRFGPANEIGVFEMRGCGLADVTNPSMFFMGDHTQVSAGSGVAVVMEGTRPLLVEVQALVTSSIFAPPRRTVN
GMDYHRLLMLLAVLDKRAGYAFGTRDVFVNIAGGLDVDEPAADLAVIASVMSGIKDQPLGNMALIGELGLTGEIRGVSHI
EQRIREAEKFGFRNCLVPEVNADSIGRTDCRIIPVKNIEEVLDFLF

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=54063 DCF50_RS09435 WP_015043975.1 1960235..1961575(-) (radA) [Dehalobacter sp. CF]
TTGGCAGGCCCAAAGACGAAGTTCTATTGCCGGGAATGCGGGCAGGAAAGCGCGAGATGGCTCGGAAGGTGTCCCGGATG
CGGTGAATGGAATACACTGATTGAGGAACGCGTAGAAAAATCCAAACCTACAGAAAACAGAGGGATCGTTCAGGCGATCC
CTCTGACTGAAATCCAAACGATGGAAGGGCAGAGACTTGATACCGGAAGTCAGGAACTTAACCGGGTTTTTGGGGGAGGG
GTTGTCGAAGGATCTTTTGTTCTTTTAAGCGGAGAGCCTGGAATCGGCAAATCTACGCTGTTTCTGCAAATGGCAGAATA
TCTTTCCCGGAAGGAAAATGTGCTTTATGTGTCGGGAGAGGAGTCTGCCCGGCAAATCAAACTCAGGGCTGACAGAATGG
AGCTTTCTTCTTCCCGGGTACATATACTCACCGATAATTCCCTGGAAGCAGTACGTTCGGAGGTGCTGAACAAAGGCTAT
AAAGTCGTTTTTATTGATTCAATTCAGACGATGCTGCTCGAAGACGTCCAGTCTGCACCCGGAAGCGTCAGCCAGGTCAG
GGAAGGGGCTTCTTTTTTGCTGAAACTGGCCAAAGAAAATGAGATTACGGTTTTCCTGGCCGGCCATATTACAAAGGAGG
GTGTGATTGCCGGACCCAGGGTTCTGGAGCACATGGTCGATACGGTCCTGTATTTTGAAGGAGATCAGCACCATATTTTC
CGTCTGCTGCGGGCAGTTAAAAACCGTTTTGGTCCTGCCAACGAAATTGGGGTATTTGAGATGAGAGGCTGCGGTCTAGC
TGATGTTACAAATCCCTCCATGTTTTTTATGGGCGATCATACACAAGTATCCGCTGGTTCCGGGGTGGCTGTCGTGATGG
AAGGAACAAGGCCGCTCTTAGTGGAAGTCCAGGCTTTGGTAACATCGTCCATCTTTGCGCCGCCGAGAAGAACCGTAAAC
GGGATGGATTACCACCGCCTGCTGATGCTTCTGGCAGTCCTGGACAAACGAGCCGGGTACGCTTTTGGCACCCGGGATGT
TTTTGTCAATATTGCCGGGGGTCTCGATGTGGATGAACCGGCAGCGGATCTTGCCGTCATCGCCTCTGTGATGTCGGGAA
TTAAAGATCAGCCGCTTGGAAATATGGCTTTAATCGGGGAACTCGGTTTGACCGGAGAAATTAGAGGGGTTTCTCATATT
GAGCAGCGGATCAGAGAAGCGGAAAAATTTGGTTTCCGAAATTGCCTGGTACCAGAAGTCAATGCAGACAGTATCGGGAG
GACCGATTGCCGGATCATTCCGGTTAAAAACATTGAGGAAGTGCTGGATTTTCTTTTTTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-214)

Subunit ChlI of Mg-chelatase (ChlI)

(337-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4LH30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.331

100

0.511
  radA Streptococcus pneumoniae Rx1

48.156

100

0.498
  radA Streptococcus pneumoniae D39

48.156

100

0.498
  radA Streptococcus pneumoniae R6

48.156

100

0.498
  radA Streptococcus pneumoniae TIGR4

48.156

100

0.498
  radA Streptococcus mitis NCTC 12261

48.156

100

0.498
  radA Streptococcus mitis SK321

48.156

100

0.498

Multiple sequence alignment