Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   JRY11_RS10570 Genome accession   NZ_CP070382
Coordinates   2062293..2063615 (+) Length   440 a.a.
NCBI ID   WP_023189341.1    Uniprot ID   A0A0V8BZU5
Organism   Lactococcus lactis strain KF140     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2057293..2068615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JRY11_RS10545 (JRY11_002122) glyQ 2057809..2058762 (-) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -
  JRY11_RS10550 (JRY11_002123) - 2059048..2059794 (-) 747 WP_023189339.1 bifunctional metallophosphatase/5'-nucleotidase -
  JRY11_RS10555 (JRY11_002124) - 2059909..2060928 (-) 1020 WP_012897709.1 DUF475 domain-containing protein -
  JRY11_RS10560 (JRY11_002125) - 2060976..2061473 (-) 498 WP_023189340.1 VanZ family protein -
  JRY11_RS10565 (JRY11_002126) - 2061607..2062236 (-) 630 WP_003130660.1 YigZ family protein -
  JRY11_RS10570 (JRY11_002127) comFA 2062293..2063615 (+) 1323 WP_023189341.1 DEAD/DEAH box helicase Machinery gene
  JRY11_RS10575 comFC 2063858..2064262 (+) 405 WP_223848637.1 ComF family protein Machinery gene
  JRY11_RS10580 (JRY11_002129) - 2064295..2064872 (-) 578 Protein_2036 nucleotidyltransferase family protein -
  JRY11_RS10585 (JRY11_002130) - 2064886..2065341 (-) 456 WP_003130653.1 diacylglycerol kinase -
  JRY11_RS10590 (JRY11_002131) ybeY 2065325..2065813 (-) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  JRY11_RS10595 (JRY11_002132) - 2065965..2066444 (-) 480 WP_003130650.1 NUDIX hydrolase -
  JRY11_RS10600 (JRY11_002133) - 2066502..2067470 (-) 969 WP_003130649.1 PhoH family protein -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50249.76 Da        Isoelectric Point: 10.1065

>NTDB_id=539619 JRY11_RS10570 WP_023189341.1 2062293..2063615(+) (comFA) [Lactococcus lactis strain KF140]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=539619 JRY11_RS10570 WP_023189341.1 2062293..2063615(+) (comFA) [Lactococcus lactis strain KF140]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACCATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0V8BZU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.029

100

0.38