Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   LJN50_RS03535 Genome accession   NZ_CP085619
Coordinates   718823..719482 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain EC32     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 713823..724482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJN50_RS03505 (LJN50_03505) ygiN 714723..715037 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  LJN50_RS03510 (LJN50_03510) mdaB 715068..715649 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  LJN50_RS03515 (LJN50_03515) - 715900..716379 (+) 480 WP_000069057.1 Hcp family type VI secretion system effector -
  LJN50_RS03520 (LJN50_03520) - 716382..717092 (+) 711 WP_000834026.1 hypothetical protein -
  LJN50_RS03525 (LJN50_03525) ygiZ 717099..717431 (+) 333 WP_000914696.1 DUF2645 family protein -
  LJN50_RS03530 (LJN50_03530) qseC 717477..718826 (-) 1350 WP_000673383.1 quorum sensing histidine kinase QseC -
  LJN50_RS03535 (LJN50_03535) ciaR 718823..719482 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  LJN50_RS03540 (LJN50_03540) ygiW 719634..720026 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  LJN50_RS03545 (LJN50_03545) ygiV 720079..720561 (+) 483 WP_000183485.1 AraC family transcriptional regulator -
  LJN50_RS03550 (LJN50_03550) ygiS 720670..722277 (+) 1608 WP_001296417.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=538522 LJN50_RS03535 WP_001221493.1 718823..719482(-) (ciaR) [Escherichia coli strain EC32]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=538522 LJN50_RS03535 WP_001221493.1 718823..719482(-) (ciaR) [Escherichia coli strain EC32]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365