Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LH369_RS03255 Genome accession   NZ_CP085060
Coordinates   681705..682145 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain P30_L1_05.20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 676705..687145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LH369_RS03230 (LH369_03230) coaE 677195..677815 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  LH369_RS03235 (LH369_03235) - 677840..677884 (+) 45 WP_120795372.1 protein YacM -
  LH369_RS03240 (LH369_03240) guaC 678040..679083 (+) 1044 WP_001217338.1 GMP reductase -
  LH369_RS03245 (LH369_03245) hofC 679118..680320 (-) 1203 WP_000157266.1 protein transport protein HofC -
  LH369_RS03250 (LH369_03250) pilB 680310..681695 (-) 1386 WP_001025146.1 type II secretion system protein GspE Machinery gene
  LH369_RS03255 (LH369_03255) pilA 681705..682145 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  LH369_RS03260 (LH369_03260) nadC 682348..683241 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LH369_RS03265 (LH369_03265) ampD 683329..683880 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LH369_RS03270 (LH369_03270) ampE 683877..684731 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  LH369_RS03275 (LH369_03275) aroP 684774..686147 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=536643 LH369_RS03255 WP_000360904.1 681705..682145(-) (pilA) [Escherichia coli strain P30_L1_05.20]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=536643 LH369_RS03255 WP_000360904.1 681705..682145(-) (pilA) [Escherichia coli strain P30_L1_05.20]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363