Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   LHL15_RS03605 Genome accession   NZ_CP084900
Coordinates   748554..749213 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain EC20     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 743554..754213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LHL15_RS03580 parE 743645..745537 (+) 1893 WP_000195292.1 DNA topoisomerase IV subunit B -
  LHL15_RS03585 ygiN 745585..745899 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  LHL15_RS03590 mdaB 745930..746511 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  LHL15_RS03595 ygiZ 746830..747162 (+) 333 WP_000917684.1 DUF2645 family protein -
  LHL15_RS03600 qseC 747208..748557 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  LHL15_RS03605 ciaR 748554..749213 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  LHL15_RS03610 ygiW 749365..749757 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  LHL15_RS03615 ygiV 749810..750292 (+) 483 WP_000183505.1 AraC family transcriptional regulator -
  LHL15_RS03620 mqsR 750497..750793 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  LHL15_RS03625 mqsA 750795..751190 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  LHL15_RS03630 ygiS 751323..752930 (+) 1608 WP_064221462.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=536357 LHL15_RS03605 WP_001221493.1 748554..749213(-) (ciaR) [Escherichia coli strain EC20]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=536357 LHL15_RS03605 WP_001221493.1 748554..749213(-) (ciaR) [Escherichia coli strain EC20]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365