Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   I6J47_RS15230 Genome accession   NZ_CP069496
Coordinates   3165959..3166711 (+) Length   250 a.a.
NCBI ID   WP_032006367.1    Uniprot ID   A0AAN4MGF9
Organism   Acinetobacter pittii strain FDAARGOS_1217     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3160959..3171711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J47_RS15215 (I6J47_15215) ponA 3161540..3164101 (-) 2562 WP_032052651.1 penicillin-binding protein PBP1a -
  I6J47_RS15220 (I6J47_15220) pilM 3164263..3165321 (+) 1059 WP_032006368.1 pilus assembly protein PilM Machinery gene
  I6J47_RS15225 (I6J47_15225) pilN 3165321..3165962 (+) 642 WP_002116944.1 PilN domain-containing protein Machinery gene
  I6J47_RS15230 (I6J47_15230) pilO 3165959..3166711 (+) 753 WP_032006367.1 type 4a pilus biogenesis protein PilO Machinery gene
  I6J47_RS15235 (I6J47_15235) pilP 3166722..3167249 (+) 528 WP_002116864.1 pilus assembly protein PilP Machinery gene
  I6J47_RS15240 (I6J47_15240) pilQ 3167312..3169438 (+) 2127 WP_017481729.1 type IV pilus secretin PilQ Machinery gene
  I6J47_RS15245 (I6J47_15245) aroK 3169450..3170019 (+) 570 WP_002116987.1 shikimate kinase AroK -
  I6J47_RS15250 (I6J47_15250) aroB 3170048..3171130 (+) 1083 WP_032006366.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28225.10 Da        Isoelectric Point: 4.8782

>NTDB_id=535098 I6J47_RS15230 WP_032006367.1 3165959..3166711(+) (pilO) [Acinetobacter pittii strain FDAARGOS_1217]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAAENPEHQEKNASASSVAP
DSSTANIQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=535098 I6J47_RS15230 WP_032006367.1 3165959..3166711(+) (pilO) [Acinetobacter pittii strain FDAARGOS_1217]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTCCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGCTCTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGTGCTGCAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACATACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

69.583

96

0.668