Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrB   Type   Machinery gene
Locus tag   LFW28_RS19420 Genome accession   NZ_CP084529
Coordinates   4029388..4031409 (-) Length   673 a.a.
NCBI ID   WP_000042533.1    Uniprot ID   Q324B3
Organism   Escherichia coli strain Origami2-DE3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4024388..4036409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFW28_RS19390 (LFW28_19390) moaE 4025188..4025640 (-) 453 WP_000852296.1 molybdopterin synthase catalytic subunit MoaE -
  LFW28_RS19395 (LFW28_19395) moaD 4025642..4025887 (-) 246 WP_000598619.1 molybdopterin synthase sulfur carrier subunit -
  LFW28_RS19400 (LFW28_19400) moaC 4025880..4026365 (-) 486 WP_000080885.1 cyclic pyranopterin monophosphate synthase MoaC -
  LFW28_RS19405 (LFW28_19405) moaB 4026368..4026880 (-) 513 WP_000084639.1 molybdenum cofactor biosynthesis protein B -
  LFW28_RS19410 (LFW28_19410) moaA 4026902..4027891 (-) 990 WP_001350494.1 GTP 3',8-cyclase MoaA -
  LFW28_RS19415 (LFW28_19415) yvcK 4028288..4029196 (+) 909 WP_001295302.1 uridine diphosphate-N-acetylglucosamine-binding protein YvcK -
  LFW28_RS19420 (LFW28_19420) uvrB 4029388..4031409 (-) 2022 WP_000042533.1 excinuclease ABC subunit UvrB Machinery gene
  LFW28_RS19425 (LFW28_19425) bioD 4031988..4032665 (-) 678 WP_000044843.1 dethiobiotin synthase -
  LFW28_RS19430 (LFW28_19430) bioC 4032658..4033413 (-) 756 WP_000246761.1 malonyl-ACP O-methyltransferase BioC -
  LFW28_RS19435 (LFW28_19435) bioF 4033400..4034554 (-) 1155 WP_000118826.1 8-amino-7-oxononanoate synthase -
  LFW28_RS19440 (LFW28_19440) bioB 4034551..4035591 (-) 1041 WP_000951213.1 biotin synthase BioB -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 76226.06 Da        Isoelectric Point: 4.8843

>NTDB_id=535074 LFW28_RS19420 WP_000042533.1 4029388..4031409(-) (uvrB) [Escherichia coli strain Origami2-DE3]
MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSGKTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEF
FPENAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFDEEVERLSLFDPLTGQIVSTIPR
FTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLENNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGE
PPPTLFDYLPADGLLVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYVSATPGNYE
LEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVLVTTLTKRMAEDLTEYLEEHGERVRYLHSDI
DTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPS
MAKAIGETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVPMDMSPKALQQKIHELE
GLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=535074 LFW28_RS19420 WP_000042533.1 4029388..4031409(-) (uvrB) [Escherichia coli strain Origami2-DE3]
ATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGG
GCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTG
CTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTC
TTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACAC
TTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGC
GTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCAT
CTCACGGTCGGTATGATTATCGATCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGC
ATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCG
TGGAACTGTTTGACGAGGAAGTGGAACGATTGTCGTTATTTGACCCGCTGACCGGGCAGATTGTTTCCACTATTCCACGT
TTTACCATCTACCCGAAAACGCACTACGTCACACCGCGCGAGCGCATCGTACAGGCGATGGAGGAGATCAAAGAAGAGCT
GGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAAACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATC
TGGAGATGATGAACGAGCTGGGCTACTGTTCGGGGATTGAAAACTACTCGCGCTTCCTCTCCGGTCGTGGACCGGGTGAG
CCACCGCCGACGCTGTTTGATTACCTGCCTGCCGATGGGCTGCTGGTCGTCGATGAATCTCACGTCACCATTCCACAAAT
TGGCGGCATGTATCGCGGTGACCGGGCGCGTAAAGAGACACTGGTGGAGTACGGCTTCCGCCTGCCATCAGCGCTGGATA
ACCGTCCGCTTAAGTTTGAAGAGTTCGAAGCATTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAG
CTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCC
GGTGGCGACACAGGTTGATGATCTTCTTTCGGAGATTCGTCAGCGAGCGGCAATTAACGAACGCGTACTGGTCACCACAC
TGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATC
GACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCG
CGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTT
CGTTGATCCAGACCATTGGTCGTGCGGCACGTAACGTTAACGGTAAAGCGATTCTCTACGGCGATAAGATCACCCCATCA
ATGGCGAAAGCGATTGGCGAAACCGAACGTCGCCGTGAGAAACAGCAGAAGTACAACGAGGAACACGGAATTACGCCGCA
AGGCTTGAACAAGAAAGTGGTCGATATCCTGGCGCTGGGGCAGAACATTGCCAAAACCAAAGCGAAGGGCAGAGGAAAAT
CGCGCCCGATTGTTGAGCCGGATAATGTGCCGATGGATATGTCGCCTAAAGCGTTGCAGCAGAAAATCCATGAGCTGGAA
GGGTTGATGATGCAACACGCGCAGAATCTGGAGTTCGAAGAAGCGGCGCAAATTCGTGACCAGTTGCATCAGCTGCGTGA
GCTGTTTATCGCGGCATCGTAA

Domains

Predicted by InterProScan.

UvrB interaction domain (UvrB_inter)

(159-249)

UvrB/uvrC motif (UVR)

(634-667)

Ultra-violet resistance protein B (UvrB)

(552-593)

Type III restriction enzyme, res subunit (ResIII)

(13-92)

Helicase conserved C-terminal domain (Helicase_C)

(436-544)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q324B3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrB Streptococcus pneumoniae TIGR4

56.372

99.108

0.559
  uvrB Streptococcus pneumoniae R6

56.222

99.108

0.557
  uvrB Streptococcus pneumoniae D39

56.222

99.108

0.557