Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LEL82_RS30240 Genome accession   NZ_CP084321
Coordinates   6467094..6467717 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain HS18-89     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6462094..6472717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEL82_RS30220 (LEL82_30180) aroB 6462689..6463795 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  LEL82_RS30225 (LEL82_30185) aroK 6463845..6464363 (-) 519 WP_003095833.1 shikimate kinase AroK -
  LEL82_RS30230 (LEL82_30190) pilQ 6464375..6466519 (-) 2145 WP_003123619.1 type 4a pilus secretin PilQ Machinery gene
  LEL82_RS30235 (LEL82_30195) comL/pilP 6466573..6467097 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  LEL82_RS30240 (LEL82_30200) pilO 6467094..6467717 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  LEL82_RS30245 (LEL82_30205) pilN 6467714..6468310 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  LEL82_RS30250 (LEL82_30210) pilM 6468310..6469377 (-) 1068 WP_087935230.1 type IV pilus assembly protein PilM Machinery gene
  LEL82_RS30255 (LEL82_30215) - 6469437..6470417 (+) 981 WP_064613796.1 IS5 family transposase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=534897 LEL82_RS30240 WP_003103268.1 6467094..6467717(-) (pilO) [Pseudomonas aeruginosa strain HS18-89]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=534897 LEL82_RS30240 WP_003103268.1 6467094..6467717(-) (pilO) [Pseudomonas aeruginosa strain HS18-89]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401