Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JR311_RS12530 Genome accession   NZ_CP069391
Coordinates   2610029..2611099 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain Sam8H1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2605029..2616099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JR311_RS12480 (JR311_12480) sipW 2605090..2605674 (-) 585 WP_012117977.1 signal peptidase I SipW -
  JR311_RS12485 (JR311_12485) tapA 2605646..2606316 (-) 671 Protein_2426 amyloid fiber anchoring/assembly protein TapA -
  JR311_RS12490 (JR311_12490) - 2606575..2606904 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  JR311_RS12495 (JR311_12495) - 2606944..2607123 (-) 180 WP_003153093.1 YqzE family protein -
  JR311_RS12500 (JR311_12500) comGG 2607180..2607557 (-) 378 WP_094247734.1 competence type IV pilus minor pilin ComGG Machinery gene
  JR311_RS12505 (JR311_12505) comGF 2607558..2608058 (-) 501 WP_014305411.1 competence type IV pilus minor pilin ComGF -
  JR311_RS12510 (JR311_12510) comGE 2607967..2608281 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  JR311_RS12515 (JR311_12515) comGD 2608265..2608702 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  JR311_RS12520 (JR311_12520) comGC 2608692..2609000 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  JR311_RS12525 (JR311_12525) comGB 2609005..2610042 (-) 1038 WP_003153086.1 competence type IV pilus assembly protein ComGB Machinery gene
  JR311_RS12530 (JR311_12530) comGA 2610029..2611099 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  JR311_RS12535 (JR311_12535) - 2611291..2612241 (-) 951 WP_204529786.1 magnesium transporter CorA family protein -
  JR311_RS12540 (JR311_12540) - 2612387..2613688 (+) 1302 WP_070082114.1 hemolysin family protein -
  JR311_RS12545 (JR311_12545) - 2613726..2614556 (-) 831 WP_070082115.1 STAS domain-containing protein -
  JR311_RS12555 (JR311_12555) - 2614762..2615142 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JR311_RS12560 (JR311_12560) - 2615379..2615624 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=534384 JR311_RS12530 WP_003153083.1 2610029..2611099(-) (comGA) [Bacillus velezensis strain Sam8H1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=534384 JR311_RS12530 WP_003153083.1 2610029..2611099(-) (comGA) [Bacillus velezensis strain Sam8H1]
GTGAAAAATATTGAAACATACAGCAGGAATATCATAAATGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374