Detailed information NTG0053395

Bioinformatically predicted

Overview

• Name: ruvA
• Type: Machinery gene
• Locus tag: U722_RS12640
• Genome accession: NC_023073
• Coordinates: 2681415..2682020 (-)
• Length: 201 a.a.
• NCBI ID: WP_003152683.1
• Uniprot ID: A7Z769
• Organism: Bacillus amyloliquefaciens LFB112
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2676415..2687020

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U722_RS12605 (U722_13445)	-	2676427..2677083 (-)	657	WP_003152699.1	DUF421 domain-containing protein	-
U722_RS12610 (U722_13450)	-	2677250..2677636 (+)	387	WP_003152697.1	TIGR04086 family membrane protein	-
U722_RS12615 (U722_13455)	yajC	2677688..2677948 (-)	261	WP_003152695.1	preprotein translocase subunit YajC	-
U722_RS12620 (U722_13460)	tgt	2677980..2679125 (-)	1146	WP_014305500.1	tRNA guanosine(34) transglycosylase Tgt	-
U722_RS12625 (U722_13465)	queA	2679153..2680181 (-)	1029	WP_003152692.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
U722_RS12630 (U722_13470)	-	2680207..2680407 (-)	201	WP_015387898.1	DUF2905 domain-containing protein	-
U722_RS12635 (U722_13475)	ruvB	2680400..2681404 (-)	1005	WP_003152685.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
U722_RS12640 (U722_13480)	ruvA	2681415..2682020 (-)	606	WP_003152683.1	Holliday junction branch migration protein RuvA	Machinery gene
U722_RS12645 (U722_13485)	-	2682155..2682664 (-)	510	WP_012118093.1	intercompartmental signaling factor BofC	-
U722_RS20475 (U722_13490)	-	2682797..2682916 (-)	120	WP_024085651.1	hypothetical protein	-
U722_RS12655 (U722_13495)	-	2683050..2683643 (-)	594	WP_003152677.1	YhcN/YlaJ family sporulation lipoprotein	-
U722_RS12660 (U722_13500)	safA	2683791..2684996 (-)	1206	WP_014305504.1	SafA/ExsA family spore coat assembly protein	-
U722_RS12665 (U722_13505)	nadA	2685123..2686226 (-)	1104	WP_024085652.1	quinolinate synthase NadA	-

Sequence

Protein

• Length: 201 a.a.
• Molecular weight: 22414.85 Da
• Isoelectric Point: 5.1597

>NTDB_id=53395 U722_RS12640 WP_003152683.1 2681415..2682020(-) (ruvA) [Bacillus amyloliquefaciens LFB112]
MIEFVKGTIDYVSPQYIVIENGGIGYQVFTPNPFIYKVSNQETIFTYHHIKEDAFSLYGFSTREEKALFTKLLNVTGIGP
KGALAILGSGDPGAVIEAIEQEDEAFLVKFPGVGKKTARQIILDLKGKLADVVPEMIDNLFNHEARIEKQEAETALDEAL
EALRVLGYAEKEIKKVLPHLKEETALSTDQYVKKALQKLLK

Nucleotide

• Length: 606 bp

>NTDB_id=53395 U722_RS12640 WP_003152683.1 2681415..2682020(-) (ruvA) [Bacillus amyloliquefaciens LFB112]
GTGATTGAATTCGTGAAGGGGACGATTGATTATGTATCTCCCCAATACATCGTGATTGAAAACGGCGGAATCGGCTATCA
GGTCTTTACTCCGAATCCGTTTATTTATAAGGTAAGCAATCAAGAAACCATTTTTACGTATCATCATATAAAAGAAGATG
CCTTTTCTTTGTACGGTTTTTCAACCCGGGAAGAAAAAGCGCTTTTCACAAAGCTGCTGAACGTCACCGGCATCGGGCCG
AAAGGGGCGCTCGCCATCCTCGGTTCCGGTGACCCCGGAGCCGTCATCGAAGCGATCGAACAGGAAGATGAAGCCTTTTT
GGTGAAATTTCCGGGTGTGGGCAAAAAAACGGCGCGGCAGATTATTCTTGATTTGAAAGGCAAACTGGCCGACGTCGTGC
CTGAAATGATTGACAACCTGTTCAATCATGAAGCGCGCATTGAAAAACAAGAGGCTGAAACGGCGCTTGACGAGGCGCTT
GAAGCGCTTAGGGTTCTCGGCTACGCCGAAAAAGAAATTAAAAAAGTGCTTCCTCACTTAAAAGAGGAAACGGCGCTTTC
AACAGATCAATATGTGAAAAAAGCATTGCAAAAATTATTAAAGTAA

Domains

Predicted by InterProScan.

• RuvA, C-terminal domain (RuvA_C) (155-199)

• RuvA N terminal domain (RuvA_N) (1-59)

• Helix-hairpin-helix domain (HHH_5) (69-126)

3D structure

• AlphaFold DB: A7Z769

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvA	Bacillus subtilis subsp. subtilis str. 168	92.537	100	0.925
ruvA	Streptococcus pneumoniae R6	39.806	100	0.408
ruvA	Streptococcus pneumoniae D39	39.806	100	0.408
ruvA	Streptococcus pneumoniae TIGR4	39.806	100	0.408