Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   LCR13_RS10655 Genome accession   NZ_CP084055
Coordinates   2162828..2163487 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain X14-3     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2157828..2168487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCR13_RS10630 (LCR13_10650) ygiS 2159111..2160718 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  LCR13_RS10635 (LCR13_10655) mqsA 2160851..2161246 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  LCR13_RS10640 (LCR13_10660) mqsR 2161248..2161544 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  LCR13_RS10645 (LCR13_10665) ygiV 2161749..2162231 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  LCR13_RS10650 (LCR13_10670) ygiW 2162284..2162676 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  LCR13_RS10655 (LCR13_10675) ciaR 2162828..2163487 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  LCR13_RS10660 (LCR13_10680) qseC 2163484..2164833 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  LCR13_RS10670 (LCR13_10690) mdaB 2165855..2166436 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  LCR13_RS10675 (LCR13_10695) ygiN 2166467..2166781 (+) 315 WP_000958598.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=533925 LCR13_RS10655 WP_001221493.1 2162828..2163487(+) (ciaR) [Escherichia coli strain X14-3]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=533925 LCR13_RS10655 WP_001221493.1 2162828..2163487(+) (ciaR) [Escherichia coli strain X14-3]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365