Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   LC440_RS02850 Genome accession   NZ_CP084026
Coordinates   570253..570912 (-) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain 035053C1pKPC2_Vir_090515B1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 565253..575912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LC440_RS02825 (LC440_02825) parE 565344..567236 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  LC440_RS02830 (LC440_02830) ygiN 567284..567598 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  LC440_RS02835 (LC440_02835) mdaB 567629..568210 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  LC440_RS02840 (LC440_02840) ygiZ 568529..568861 (+) 333 WP_000917684.1 DUF2645 family protein -
  LC440_RS02845 (LC440_02845) qseC 568907..570256 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  LC440_RS02850 (LC440_02850) ciaR 570253..570912 (-) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  LC440_RS02855 (LC440_02855) ygiW 571064..571456 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  LC440_RS02860 (LC440_02860) ygiV 571509..571991 (+) 483 WP_000183505.1 AraC family transcriptional regulator -
  LC440_RS02865 (LC440_02865) mqsR 572196..572492 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  LC440_RS02870 (LC440_02870) mqsA 572494..572889 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  LC440_RS02875 (LC440_02875) ygiS 573022..574629 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=533827 LC440_RS02850 WP_001221495.1 570253..570912(-) (ciaR) [Escherichia coli strain 035053C1pKPC2_Vir_090515B1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=533827 LC440_RS02850 WP_001221495.1 570253..570912(-) (ciaR) [Escherichia coli strain 035053C1pKPC2_Vir_090515B1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361