Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   U722_RS08475 Genome accession   NC_023073
Coordinates   1741025..1742902 (+) Length   625 a.a.
NCBI ID   WP_003154106.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens LFB112     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1736025..1747902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U722_RS08460 (U722_08920) ymcA 1737075..1737506 (+) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  U722_RS08465 (U722_08925) cotE 1737760..1738305 (+) 546 WP_003154111.1 outer spore coat protein CotE -
  U722_RS08470 (U722_08930) hexA 1738424..1741009 (+) 2586 WP_024085312.1 DNA mismatch repair protein MutS Machinery gene
  U722_RS08475 (U722_08935) hexB 1741025..1742902 (+) 1878 WP_003154106.1 DNA mismatch repair endonuclease MutL Machinery gene
  U722_RS08480 (U722_08940) - 1743469..1744113 (+) 645 WP_024085313.1 hypothetical protein -
  U722_RS08485 - 1744305..1744727 (+) 423 WP_041482363.1 hypothetical protein -
  U722_RS20020 - 1744879..1745212 (+) 334 Protein_1650 HNH endonuclease -
  U722_RS20380 - 1745340..1745525 (+) 186 Protein_1651 hypothetical protein -
  U722_RS08495 (U722_08955) - 1745812..1746021 (-) 210 WP_024085315.1 hypothetical protein -
  U722_RS08500 (U722_08960) - 1746655..1747332 (+) 678 WP_024085316.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 625 a.a.        Molecular weight: 70230.35 Da        Isoelectric Point: 5.7702

>NTDB_id=53364 U722_RS08475 WP_003154106.1 1741025..1742902(+) (hexB) [Bacillus amyloliquefaciens LFB112]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDANSTVIEIDIEEAGLSSIRVLDNGEGMETDDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEIKTSIGEGAGTHLILQGGNIISEQKSSSRKGTEIIVTNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHQGKNLLQTNGNGDVRHVLAAIYGTAVAKKMIPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVINGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHELIRDG
IKEVFQKQRLIPSAQLPKKTAPAPAKNEQQFMTFEESAPERKLPEKTPEPSYSPMKLSSVVKEPEPVIEEESLPDEAGHG
VPPELGMPVTEAPVSDIPEPEEEADSEEIREERVPVMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVGQV
EPEVQDMIVPLTFHYAANEALIIDQHQHELESVGVFLEAFGTNSYIVRCHPAWFPKGEEAELIEEIIQQVLDAKQIDIKK
LREEAAIMMSCKGSIKANRHLRNDEIKALLDDLRRTTDPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1878 bp        

>NTDB_id=53364 U722_RS08475 WP_003154106.1 1741025..1742902(+) (hexB) [Bacillus amyloliquefaciens LFB112]
TTGGCAAAAGTCATCCAGCTGTCAGATGAACTTTCGAATAAAATAGCGGCCGGCGAGGTGGTCGAACGGCCTGCCTCAGT
CGTTAAAGAATTGGTGGAAAACGCCATTGACGCAAACAGCACAGTCATTGAGATCGACATAGAGGAAGCGGGGCTTTCGT
CGATTCGGGTGCTCGATAACGGCGAAGGGATGGAAACCGATGATTGCAAGCGGGCTTTCCGCCGCCACGCGACCAGTAAA
ATCAAAGATGAAAACGACCTGTTCCGGGTGAGAACACTCGGTTTCCGCGGAGAAGCTTTGCCAAGTATCGCGTCTGTTTC
TCATCTGGAAATCAAAACGAGCATCGGAGAGGGGGCGGGAACCCATCTCATTCTTCAGGGCGGGAATATCATTTCTGAGC
AGAAATCCTCCAGCAGAAAAGGAACGGAGATTATCGTTACCAACCTGTTTTTCAATACGCCCGCCAGATTGAAATATATG
AAAACCGTGCATACGGAGCTCGGCAATATTACGGATGTTGTCAACCGGATTGCTCTTGCGCATCCGGAAGTATCCATCCG
TCTGCGCCATCAGGGGAAAAATCTGTTGCAGACAAACGGTAACGGGGACGTGCGCCATGTGCTGGCCGCAATTTACGGGA
CGGCGGTTGCGAAAAAAATGATACCGCTCCATGTCAGCTCGCTTGATTTTGAAGTCAAGGGCTACATCGCGCTTCCGGAA
ATCACGCGTGCATCCAGAAACTACATGTCATCTGTCATTAACGGCAGATACATTAAGAATTTTCCGCTTGTAAAAGCGGT
GCATGAGGGCTATCACACACTTCTGCCGATCGGCCGCCATCCGATTACGTTTATCGAAATTACGATGGACCCGATTTTGG
TCGATGTCAATGTCCATCCGTCGAAGCTTGAAGTTCGCCTCAGCAAAGAGACGGAGCTGCATGAGCTGATCCGTGACGGG
ATAAAGGAAGTGTTTCAAAAGCAGCGGCTTATTCCGAGCGCCCAGCTCCCGAAAAAAACAGCGCCGGCACCGGCCAAAAA
CGAACAGCAATTTATGACATTCGAGGAAAGCGCGCCTGAGCGGAAACTTCCTGAAAAAACGCCGGAGCCGTCTTACTCAC
CGATGAAGCTGAGTTCGGTCGTAAAAGAGCCGGAGCCGGTCATAGAAGAAGAGTCCCTGCCGGATGAAGCAGGTCACGGA
GTGCCGCCGGAGCTCGGCATGCCGGTAACGGAAGCGCCTGTTTCCGATATTCCGGAGCCTGAAGAGGAGGCTGACTCTGA
AGAAATACGGGAAGAAAGAGTTCCGGTGATGTATCCGATCGGCCAAATGCACGGGACGTATATTTTAGCCCAAAACGAAA
ACGGCTTGTATATCATTGACCAGCATGCGGCGCAAGAACGGATTAAATACGAATATTTCCGGGAGAAAGTCGGGCAGGTC
GAGCCTGAAGTGCAGGATATGATCGTGCCGCTCACCTTTCATTATGCGGCAAATGAAGCGCTGATCATTGATCAGCATCA
GCATGAGCTTGAAAGCGTCGGAGTATTTTTAGAAGCATTCGGCACGAACAGCTACATCGTCCGCTGCCATCCGGCCTGGT
TTCCAAAAGGGGAAGAAGCTGAACTGATTGAAGAAATCATTCAGCAGGTGCTTGACGCCAAACAGATCGATATTAAAAAA
CTCAGGGAGGAAGCGGCCATTATGATGAGCTGCAAAGGCTCAATCAAAGCGAACCGCCATTTGAGAAATGACGAAATCAA
AGCGCTCTTGGACGACCTGCGCCGAACGACAGACCCGTTCACATGCCCGCACGGCCGGCCAATCATTATCCACCACAGTA
CGTATGAGATGGAGAAGATGTTTAAGCGTGTGATGTAG

Domains

Predicted by InterProScan.

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-325)

MutL C terminal dimerisation domain (MutL_C)

(440-581)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-111)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

46.522

100

0.482

Multiple sequence alignment