Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   LA372_RS03495 Genome accession   NZ_CP083585
Coordinates   701356..702015 (-) Length   219 a.a.
NCBI ID   WP_021574775.1    Uniprot ID   -
Organism   Escherichia coli strain ECNB21-M121     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 696356..707015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA372_RS03465 (LA372_03465) - 696472..697419 (+) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -
  LA372_RS03470 (LA372_03470) - 697416..698303 (+) 888 WP_000614954.1 MurR/RpiR family transcriptional regulator -
  LA372_RS03475 (LA372_03475) ygiN 698348..698662 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  LA372_RS03480 (LA372_03480) mdaB 698693..699274 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  LA372_RS03485 (LA372_03485) ygiZ 699632..699964 (+) 333 WP_024171565.1 DUF2645 family protein -
  LA372_RS03490 (LA372_03490) qseC 700010..701359 (-) 1350 WP_047624819.1 quorum sensing histidine kinase QseC -
  LA372_RS03495 (LA372_03495) ciaR 701356..702015 (-) 660 WP_021574775.1 quorum sensing response regulator transcription factor QseB Regulator
  LA372_RS03500 (LA372_03500) ygiW 702167..702559 (+) 393 WP_047624822.1 OB fold stress tolerance protein YgiW -
  LA372_RS03505 (LA372_03505) ygiV 702612..703094 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  LA372_RS03510 (LA372_03510) ygiS 703203..704810 (+) 1608 WP_047624824.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24704.67 Da        Isoelectric Point: 6.3527

>NTDB_id=532719 LA372_RS03495 WP_021574775.1 701356..702015(-) (ciaR) [Escherichia coli strain ECNB21-M121]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGCQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLILKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=532719 LA372_RS03495 WP_021574775.1 701356..702015(-) (ciaR) [Escherichia coli strain ECNB21-M121]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTTGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAATACTGAAACCAAAAGAATTTGCTCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAATTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365