Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   MHHHKEFG_RS18950 Genome accession   NZ_CP069125
Coordinates   3681639..3682184 (-) Length   181 a.a.
NCBI ID   WP_034660649.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 3676639..3687184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS18925 (MHHHKEFG_03863) - 3676903..3677511 (-) 609 WP_060597699.1 alpha/beta hydrolase -
  MHHHKEFG_RS18930 (MHHHKEFG_03864) - 3677508..3678479 (-) 972 WP_066031466.1 ring-cleaving dioxygenase -
  MHHHKEFG_RS18935 (MHHHKEFG_03865) - 3678671..3680188 (-) 1518 WP_066031465.1 ABC transporter permease/substrate-binding protein -
  MHHHKEFG_RS18940 (MHHHKEFG_03866) - 3680181..3681146 (-) 966 WP_066031464.1 ABC transporter ATP-binding protein -
  MHHHKEFG_RS18945 (MHHHKEFG_03867) rpsR 3681359..3681598 (-) 240 WP_008347556.1 30S ribosomal protein S18 -
  MHHHKEFG_RS18950 (MHHHKEFG_03868) ssbA 3681639..3682184 (-) 546 WP_034660649.1 single-stranded DNA-binding protein SsbA Machinery gene
  MHHHKEFG_RS18955 (MHHHKEFG_03869) rpsF 3682221..3682508 (-) 288 WP_003215073.1 30S ribosomal protein S6 -
  MHHHKEFG_RS18960 (MHHHKEFG_03870) ychF 3682617..3683717 (-) 1101 WP_034660650.1 redox-regulated ATPase YchF -
  MHHHKEFG_RS18965 (MHHHKEFG_03871) - 3683934..3684140 (-) 207 WP_012011910.1 DUF951 domain-containing protein -
  MHHHKEFG_RS18970 (MHHHKEFG_03872) - 3684435..3685754 (+) 1320 WP_066031463.1 hydrolase -
  MHHHKEFG_RS18975 (MHHHKEFG_03873) - 3685770..3686180 (+) 411 WP_060597704.1 hypothetical protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 19733.28 Da        Isoelectric Point: 4.6109

>NTDB_id=532416 MHHHKEFG_RS18950 WP_034660649.1 3681639..3682184(-) (ssbA) [Bacillus pumilus strain D5]
MLNRVVLVGRLTKDPELRYTPNGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKSGGAGSGGYSGGGNSGGQYYGGSQNDQNPFGNEPNQNPYGNQSNQQNRNQGNSF
NDDPFANDGKPIDISDDDLPF

Nucleotide


Download         Length: 546 bp        

>NTDB_id=532416 MHHHKEFG_RS18950 WP_034660649.1 3681639..3682184(-) (ssbA) [Bacillus pumilus strain D5]
ATGCTAAACCGTGTTGTATTGGTCGGAAGACTAACAAAAGACCCTGAGCTTCGCTACACGCCTAATGGTGCGGCTGTAGC
CACGTTTACTCTAGCTGTGAATCGTACGTTTACGAATCAATCTGGAGAGCGTGAAGCCGATTTCATTAACTGTGTTACTT
GGAGAAGACAAGCCGAGAACGTGGCTAATTTCTTGAAAAAAGGAAGTCTTGCCGGTGTAGACGGTCGTTTGCAGACAAGA
AACTATGAAAATCAGCAAGGACAGCGTGTCTTCGTGACAGAGGTTCAAGCTGAAAGTGTTCAATTTCTTGAGCCTAAGAG
CGGCGGTGCTGGTTCAGGTGGATACAGCGGCGGTGGCAATAGCGGAGGCCAGTATTATGGCGGTAGCCAAAACGATCAGA
ATCCATTCGGAAATGAACCAAATCAAAATCCATATGGCAATCAGAGCAACCAACAAAATCGTAATCAGGGAAATAGCTTC
AATGATGACCCATTTGCGAATGATGGTAAACCGATTGACATCTCCGATGATGATTTGCCATTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

84.53

100

0.845
  ssb Latilactobacillus sakei subsp. sakei 23K

55.08

100

0.569
  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

58.564

0.376