Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MHHHKEFG_RS16545 Genome accession   NZ_CP069125
Coordinates   3188745..3190124 (-) Length   459 a.a.
NCBI ID   WP_142393300.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3183745..3195124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS16510 (MHHHKEFG_03369) flgL 3183925..3184836 (-) 912 WP_066031682.1 flagellar hook-associated protein FlgL -
  MHHHKEFG_RS16515 (MHHHKEFG_03370) flgK 3184846..3186366 (-) 1521 WP_060597394.1 flagellar hook-associated protein FlgK -
  MHHHKEFG_RS16520 (MHHHKEFG_03371) - 3186382..3186864 (-) 483 WP_034660146.1 flagellar protein FlgN -
  MHHHKEFG_RS16525 (MHHHKEFG_03372) flgM 3186883..3187146 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  MHHHKEFG_RS16530 (MHHHKEFG_03373) - 3187226..3187645 (-) 420 WP_066031681.1 TIGR03826 family flagellar region protein -
  MHHHKEFG_RS16535 (MHHHKEFG_03374) comFC 3187702..3188388 (-) 687 WP_189288297.1 ComF family protein Machinery gene
  MHHHKEFG_RS16540 (MHHHKEFG_03375) - 3188385..3188678 (-) 294 WP_060597398.1 late competence development ComFB family protein -
  MHHHKEFG_RS16545 (MHHHKEFG_03376) comFA 3188745..3190124 (-) 1380 WP_142393300.1 DEAD/DEAH box helicase Machinery gene
  MHHHKEFG_RS16550 (MHHHKEFG_03377) - 3190245..3191087 (-) 843 WP_034660152.1 DegV family protein -
  MHHHKEFG_RS16555 (MHHHKEFG_03378) degU 3191305..3191994 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MHHHKEFG_RS16560 (MHHHKEFG_03379) degS 3192059..3193234 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  MHHHKEFG_RS16565 (MHHHKEFG_03380) - 3193456..3194124 (+) 669 WP_012011434.1 YigZ family protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52604.81 Da        Isoelectric Point: 10.2540

>NTDB_id=532409 MHHHKEFG_RS16545 WP_142393300.1 3188745..3190124(-) (comFA) [Bacillus pumilus strain D5]
MNMDKAMELRQQLQSRHLLTAETRCSQSDLDWLVEKGLVIRTPAIERKANGLTCCRCGVSKKRYFAHTPCDMCQKDCVYC
RSCIMMGKTTECGFLYEWTGPQMEETCHTELTWQGNLSKGQKRASEKLIEAIKNKFDLLIWAVCGAGKTEVLFHGIEYAL
HQGMSVCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIAPLVIATTHQLMRYKLAFDVLIIDEVDAFPYSIDE
RLQFAVLKAMRKKGVRVYLSATPSKKMKRDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNWMQK
HITKKRRILLFVPSISTMKKITKVLREHRLNVEGVSADDPDRKQKVQHFRDYKYDVLVTTTILERGVTIPNVQVGVLGAE
STIFTESALVQISGRAGRHPDFFKGDVIFFHFGLTRSMKQAKKHIVKMNDTAAKEFSEK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=532409 MHHHKEFG_RS16545 WP_142393300.1 3188745..3190124(-) (comFA) [Bacillus pumilus strain D5]
TTGAACATGGACAAAGCAATGGAACTAAGGCAGCAACTGCAGTCACGCCACCTGCTTACCGCTGAAACAAGATGCTCACA
GTCCGATTTGGATTGGCTGGTGGAGAAAGGGTTAGTCATCCGAACACCTGCTATTGAGAGGAAAGCAAATGGTCTTACTT
GCTGTAGATGTGGTGTATCAAAAAAGCGGTACTTTGCCCACACTCCGTGTGACATGTGTCAAAAAGATTGTGTGTACTGT
AGATCATGCATCATGATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAACATG
TCATACGGAATTAACATGGCAGGGAAACCTGTCTAAAGGACAAAAAAGGGCGTCAGAAAAATTGATCGAAGCGATAAAAA
ACAAATTTGATCTACTCATTTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATTGAATATGCCTTA
CATCAAGGAATGAGCGTCTGCATTGCGACACCTAGAACAGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCATT
TCAAGGAATGAAAATAGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGCACCGCTAGTGATCGCCACAACTC
ATCAGTTGATGAGATACAAACTTGCATTTGATGTGCTTATTATAGATGAAGTCGACGCCTTTCCTTATTCAATCGATGAA
CGTCTCCAATTTGCTGTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCCTCTAAGAAAAT
GAAAAGAGATGTATCTCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCGTTACCTGTCCCGT
CCTTTCAATGGATTGGACATTGGAAGAAGAAATTAAAAAAGAACCAGCTCCCACCTAAAGTAATGAATTGGATGCAGAAA
CATATCACAAAGAAGAGAAGGATTTTACTTTTTGTTCCTTCTATTTCGACCATGAAGAAGATAACGAAGGTCCTTAGAGA
ACACCGCTTAAATGTGGAGGGGGTGTCTGCTGATGATCCAGACAGGAAACAAAAGGTCCAGCACTTCAGAGATTATAAAT
ACGATGTACTAGTTACAACCACCATTCTAGAAAGAGGCGTTACCATTCCAAATGTTCAAGTGGGGGTACTAGGTGCGGAA
TCTACTATCTTTACAGAGAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCCCGATTTTTTTAAAGGAGATGT
TATCTTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGTAAAAATGAATGATACAGCTGCAA
AAGAGTTTTCTGAAAAATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(320-419)

Type III restriction enzyme, res subunit (ResIII)

(116-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

54.084

98.693

0.534