Detailed information NTG0532391

Bioinformatically predicted

Overview

• Name: comGC
• Type: Machinery gene
• Locus tag: MHHHKEFG_RS10970
• Genome accession: NZ_CP069125
• Coordinates: 2170710..2171003 (-)
• Length: 97 a.a.
• NCBI ID: WP_066030665.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain D5
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2165710..2176003

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MHHHKEFG_RS10920 (MHHHKEFG_02234)	sinR	2165874..2166209 (+)	336	WP_095285385.1	transcriptional regulator SinR	Regulator
MHHHKEFG_RS10925 (MHHHKEFG_02235)	tasA	2166277..2167077 (-)	801	WP_034661283.1	biofilm matrix protein TasA	-
MHHHKEFG_RS10930 (MHHHKEFG_02236)	sipW	2167135..2167707 (-)	573	WP_066031752.1	signal peptidase I SipW	-
MHHHKEFG_RS10935 (MHHHKEFG_02237)	tapA	2167759..2168286 (-)	528	WP_060596834.1	amyloid fiber anchoring/assembly protein TapA	-
MHHHKEFG_RS10940 (MHHHKEFG_02238)	-	2168586..2168864 (+)	279	WP_223250494.1	DUF3889 domain-containing protein	-
MHHHKEFG_RS10945 (MHHHKEFG_02239)	-	2168898..2169092 (-)	195	WP_024718846.1	YqzE family protein	-
MHHHKEFG_RS10950 (MHHHKEFG_02240)	-	2169151..2169534 (-)	384	WP_034661288.1	competence type IV pilus minor pilin ComGG	-
MHHHKEFG_RS10955 (MHHHKEFG_02241)	comGF	2169531..2170031 (-)	501	WP_034661289.1	competence type IV pilus minor pilin ComGF	-
MHHHKEFG_RS10960	comGE	2169970..2170287 (-)	318	WP_034661291.1	competence type IV pilus minor pilin ComGE	-
MHHHKEFG_RS10965 (MHHHKEFG_02242)	comGD	2170271..2170717 (-)	447	WP_066030666.1	competence type IV pilus minor pilin ComGD	-
MHHHKEFG_RS10970 (MHHHKEFG_02243)	comGC	2170710..2171003 (-)	294	WP_066030665.1	competence type IV pilus major pilin ComGC	Machinery gene
MHHHKEFG_RS10975 (MHHHKEFG_02244)	comGB	2171020..2172060 (-)	1041	WP_066030664.1	competence type IV pilus assembly protein ComGB	-
MHHHKEFG_RS10980 (MHHHKEFG_02245)	comGA	2172041..2173111 (-)	1071	WP_066030663.1	competence type IV pilus ATPase ComGA	Machinery gene
MHHHKEFG_RS10985 (MHHHKEFG_02247)	-	2173289..2174161 (-)	873	WP_034661297.1	STAS domain-containing protein	-
MHHHKEFG_RS10995 (MHHHKEFG_02249)	-	2174385..2174765 (-)	381	WP_007501232.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
MHHHKEFG_RS11000 (MHHHKEFG_02250)	-	2174979..2175221 (+)	243	WP_003217440.1	DUF2626 domain-containing protein	-
MHHHKEFG_RS11005 (MHHHKEFG_02251)	-	2175270..2175908 (-)	639	WP_060596838.1	MBL fold metallo-hydrolase	-

Sequence

Protein

• Length: 97 a.a.
• Molecular weight: 10741.62 Da
• Isoelectric Point: 6.9665

>NTDB_id=532391 MHHHKEFG_RS10970 WP_066030665.1 2170710..2171003(-) (comGC) [Bacillus pumilus strain D5]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQTIQSKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKNGLT
CPNGKAIVIQNGHVKHE

Nucleotide

• Length: 294 bp

>NTDB_id=532391 MHHHKEFG_RS10970 WP_066030665.1 2170710..2171003(-) (comGC) [Bacillus pumilus strain D5]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATTGTCATGCTCGTGATTTCGATTCTTTTACTTATTACCATCCC
GAATATCACTCGTCACCACCAAACGATTCAATCAAAGGGCTGTGAAGGGCTTAAATCAATGGTGAGCACACAGGTGATGG
CATATGAATTAGAGCATGATGGCAAGACACCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAATGGATTAACG
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCCATGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (2-25)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGC	Bacillus subtilis subsp. subtilis str. 168	67.01	100	0.67
comGC	Staphylococcus aureus MW2	42.391	94.845	0.402
comGC	Staphylococcus aureus N315	42.391	94.845	0.402