Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHHHKEFG_RS10980 Genome accession   NZ_CP069125
Coordinates   2172041..2173111 (-) Length   356 a.a.
NCBI ID   WP_066030663.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2167041..2178111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS10930 (MHHHKEFG_02236) sipW 2167135..2167707 (-) 573 WP_066031752.1 signal peptidase I SipW -
  MHHHKEFG_RS10935 (MHHHKEFG_02237) tapA 2167759..2168286 (-) 528 WP_060596834.1 amyloid fiber anchoring/assembly protein TapA -
  MHHHKEFG_RS10940 (MHHHKEFG_02238) - 2168586..2168864 (+) 279 WP_223250494.1 DUF3889 domain-containing protein -
  MHHHKEFG_RS10945 (MHHHKEFG_02239) - 2168898..2169092 (-) 195 WP_024718846.1 YqzE family protein -
  MHHHKEFG_RS10950 (MHHHKEFG_02240) - 2169151..2169534 (-) 384 WP_034661288.1 competence type IV pilus minor pilin ComGG -
  MHHHKEFG_RS10955 (MHHHKEFG_02241) comGF 2169531..2170031 (-) 501 WP_034661289.1 competence type IV pilus minor pilin ComGF -
  MHHHKEFG_RS10960 comGE 2169970..2170287 (-) 318 WP_034661291.1 competence type IV pilus minor pilin ComGE -
  MHHHKEFG_RS10965 (MHHHKEFG_02242) comGD 2170271..2170717 (-) 447 WP_066030666.1 competence type IV pilus minor pilin ComGD -
  MHHHKEFG_RS10970 (MHHHKEFG_02243) comGC 2170710..2171003 (-) 294 WP_066030665.1 competence type IV pilus major pilin ComGC Machinery gene
  MHHHKEFG_RS10975 (MHHHKEFG_02244) comGB 2171020..2172060 (-) 1041 WP_066030664.1 competence type IV pilus assembly protein ComGB -
  MHHHKEFG_RS10980 (MHHHKEFG_02245) comGA 2172041..2173111 (-) 1071 WP_066030663.1 competence type IV pilus ATPase ComGA Machinery gene
  MHHHKEFG_RS10985 (MHHHKEFG_02247) - 2173289..2174161 (-) 873 WP_034661297.1 STAS domain-containing protein -
  MHHHKEFG_RS10995 (MHHHKEFG_02249) - 2174385..2174765 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHHHKEFG_RS11000 (MHHHKEFG_02250) - 2174979..2175221 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHHHKEFG_RS11005 (MHHHKEFG_02251) - 2175270..2175908 (-) 639 WP_060596838.1 MBL fold metallo-hydrolase -
  MHHHKEFG_RS11010 (MHHHKEFG_02252) - 2176066..2176239 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHHHKEFG_RS11015 (MHHHKEFG_02253) - 2176264..2176578 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -
  MHHHKEFG_RS11020 (MHHHKEFG_02254) - 2176656..2177909 (-) 1254 WP_060596687.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40325.66 Da        Isoelectric Point: 8.8741

>NTDB_id=532392 MHHHKEFG_RS10980 WP_066030663.1 2172041..2173111(-) (comGA) [Bacillus pumilus strain D5]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASIRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDLIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFDVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRSVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=532392 MHHHKEFG_RS10980 WP_066030663.1 2172041..2173111(-) (comGA) [Bacillus pumilus strain D5]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
AAAAGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGGTCACTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGGATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCATCAGATTATTATCATTTTTGA
ATCATTCGCATGGGCTGATTTTATTTACCGGTCCAACAAATTCAGGGAAAACAACAACCCTTTATTCATTAATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGTGCTATTTTAAGACATGATCCAGATCTGATTGTGCTAG
GAGAAATAAGAGATGCCGAAACGGCCCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACGCTTCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGATGTCACGATGAATGAACTCGAACAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAT
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTAGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

63.842

99.438

0.635