Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JM956_RS22250 Genome accession   NZ_CP068719
Coordinates   4348287..4349330 (-) Length   347 a.a.
NCBI ID   WP_202822281.1    Uniprot ID   -
Organism   Bacillus cereus strain 21155     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4343287..4354330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JM956_RS22205 - 4343721..4343921 (-) 201 WP_000106083.1 YqzE family protein -
  JM956_RS22210 - 4343960..4344457 (-) 498 WP_000836648.1 shikimate kinase -
  JM956_RS22215 - 4344572..4345222 (-) 651 WP_000183865.1 2OG-Fe(II) oxygenase -
  JM956_RS22220 comGG 4345397..4345768 (-) 372 WP_001231319.1 competence type IV pilus minor pilin ComGG -
  JM956_RS22225 comGF 4345765..4346235 (-) 471 WP_000923105.1 competence type IV pilus minor pilin ComGF -
  JM956_RS22230 - 4346205..4346507 (-) 303 WP_000829463.1 hypothetical protein -
  JM956_RS22235 comGD 4346500..4346955 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  JM956_RS22240 comGC 4346952..4347251 (-) 300 WP_001178685.1 competence type IV pilus major pilin ComGC -
  JM956_RS22245 comGB 4347263..4348300 (-) 1038 WP_002164182.1 competence type IV pilus assembly protein ComGB -
  JM956_RS22250 comGA 4348287..4349330 (-) 1044 WP_202822281.1 competence type IV pilus ATPase ComGA Machinery gene
  JM956_RS22255 - 4349536..4350231 (+) 696 WP_000434104.1 helix-turn-helix domain-containing protein -
  JM956_RS22260 - 4350357..4350599 (+) 243 WP_000440717.1 DUF2626 domain-containing protein -
  JM956_RS22265 - 4350707..4352101 (+) 1395 WP_001094346.1 L-cystine transporter -
  JM956_RS22270 - 4352180..4352572 (-) 393 WP_000875397.1 hypothetical protein -
  JM956_RS22275 - 4352660..4352875 (-) 216 WP_001008332.1 DUF3912 family protein -
  JM956_RS22280 - 4353128..4353439 (+) 312 WP_001093237.1 hypothetical protein -
  JM956_RS22285 - 4353473..4353961 (-) 489 WP_000764269.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39166.53 Da        Isoelectric Point: 8.5415

>NTDB_id=530704 JM956_RS22250 WP_202822281.1 4348287..4349330(-) (comGA) [Bacillus cereus strain 21155]
MNGIEIFANAILKEACRVQASDLHIVPRQNDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIIKLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=530704 JM956_RS22250 WP_202822281.1 4348287..4349330(-) (comGA) [Bacillus cereus strain 21155]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAATGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTTGTTATTCGCCT
CCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACAGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGCCGCATCATTAAACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATACGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTGGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556
  pilB Haemophilus influenzae 86-028NP

39.441

92.795

0.366
  pilF Thermus thermophilus HB27

42.808

84.15

0.36