Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JHT21_RS13235 Genome accession   NZ_CP068638
Coordinates   2773026..2773619 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain VP120     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2768026..2778619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHT21_RS13215 (JHT21_13180) aroB 2768902..2770002 (-) 1101 WP_005496576.1 3-dehydroquinate synthase -
  JHT21_RS13220 (JHT21_13185) aroK 2770034..2770552 (-) 519 WP_005381319.1 shikimate kinase AroK -
  JHT21_RS13225 (JHT21_13190) pilQ 2770741..2772489 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  JHT21_RS13230 (JHT21_13195) pilP 2772521..2773036 (-) 516 WP_017449464.1 pilus assembly protein PilP Machinery gene
  JHT21_RS13235 (JHT21_13200) pilO 2773026..2773619 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  JHT21_RS13240 (JHT21_13205) pilN 2773612..2774190 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  JHT21_RS13245 (JHT21_13210) pilM 2774174..2775190 (-) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  JHT21_RS13250 (JHT21_13215) - 2775333..2777903 (+) 2571 WP_029804285.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=530131 JHT21_RS13235 WP_005480482.1 2773026..2773619(-) (pilO) [Vibrio parahaemolyticus strain VP120]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=530131 JHT21_RS13235 WP_005480482.1 2773026..2773619(-) (pilO) [Vibrio parahaemolyticus strain VP120]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726