Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B73_RS20225 Genome accession   NZ_CP082827
Coordinates   4189128..4189568 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SCAID WND2-2021 (3/145)     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4184128..4194568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B73_RS20205 (K8B73_20205) aroP 4185126..4186499 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  K8B73_RS20210 (K8B73_20210) ampE 4186542..4187396 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  K8B73_RS20215 (K8B73_20215) ampD 4187393..4187944 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B73_RS20220 (K8B73_20220) nadC 4188032..4188925 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B73_RS20225 (K8B73_20225) pilA 4189128..4189568 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  K8B73_RS20230 (K8B73_20230) pilB 4189578..4190963 (+) 1386 WP_001025168.1 type II secretion system protein GspE Machinery gene
  K8B73_RS20235 (K8B73_20235) hofC 4190953..4192155 (+) 1203 WP_000157287.1 protein transport protein HofC -
  K8B73_RS20240 (K8B73_20240) guaC 4192190..4193233 (-) 1044 WP_001217337.1 GMP reductase -
  K8B73_RS20245 (K8B73_20245) - 4193389..4193433 (-) 45 WP_120795372.1 protein YacM -
  K8B73_RS20250 (K8B73_20250) coaE 4193458..4194078 (+) 621 WP_001269513.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=530131 K8B73_RS20225 WP_000360904.1 4189128..4189568(+) (pilA) [Escherichia coli strain SCAID WND2-2021 (3/145)]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=530131 K8B73_RS20225 WP_000360904.1 4189128..4189568(+) (pilA) [Escherichia coli strain SCAID WND2-2021 (3/145)]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363