Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JHS83_RS13805 Genome accession   NZ_CP068622
Coordinates   2956888..2957481 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain LH24     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2951888..2962481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHS83_RS13785 (JHS83_13760) aroB 2952764..2953864 (-) 1101 WP_025546399.1 3-dehydroquinate synthase -
  JHS83_RS13790 (JHS83_13765) aroK 2953896..2954414 (-) 519 WP_005381319.1 shikimate kinase AroK -
  JHS83_RS13795 (JHS83_13770) pilQ 2954603..2956351 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  JHS83_RS13800 (JHS83_13775) pilP 2956383..2956898 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  JHS83_RS13805 (JHS83_13780) pilO 2956888..2957481 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  JHS83_RS13810 (JHS83_13785) pilN 2957474..2958052 (-) 579 WP_140391938.1 PilN domain-containing protein Machinery gene
  JHS83_RS13815 (JHS83_13790) pilM 2958036..2959052 (-) 1017 WP_031778293.1 type IV pilus biogenesis protein PilM Machinery gene
  JHS83_RS13820 (JHS83_13795) - 2959195..2961765 (+) 2571 WP_140222517.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=529998 JHS83_RS13805 WP_005480482.1 2956888..2957481(-) (pilO) [Vibrio parahaemolyticus strain LH24]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=529998 JHS83_RS13805 WP_005480482.1 2956888..2957481(-) (pilO) [Vibrio parahaemolyticus strain LH24]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CGCTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726